ExplorEnz: EC 1.14.14.158

Your query returned 1 entry. Printable version

1.14.14.158

Accepted name:

carotenoid ε hydroxylase

Reaction:

(1) α-carotene + [reduced NADPH-hemoprotein reductase] + O₂ = α-cryptoxanthin + [oxidized NADPH-hemoprotein reductase] + H₂O
(2) zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O₂ = lutein + [oxidized NADPH-hemoprotein reductase] + H₂O

For diagram of lutein biosynthesis, click here

Other name(s):

CYP97C1; LUT1; CYP97C; carotene ε-monooxygenase

Systematic name:

α-carotene,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (3-hydroxylating)

Comments:

A cytochrome P-450 (heme-thiolate) protein.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB

References:

1.	Pogson, B., McDonald, K.A., Truong, M., Britton, G. and DellaPenna, D. Arabidopsis carotenoid mutants demonstrate that lutein is not essential for photosynthesis in higher plants. Plant Cell 8 (1996) 1627–1639. [DOI] [PMID: 8837513]
2.	Tian, L., Musetti, V., Kim, J., Magallanes-Lundback, M. and DellaPenna, D. The Arabidopsis LUT1 locus encodes a member of the cytochrome P₄₅₀ family that is required for carotenoid ε-ring hydroxylation activity. Proc. Natl. Acad. Sci. USA 101 (2004) 402–407. [DOI] [PMID: 14709673]
3.	Stigliani, A.L., Giorio, G. and D'Ambrosio, C. Characterization of P450 carotenoid β- and ε-hydroxylases of tomato and transcriptional regulation of xanthophyll biosynthesis in root, leaf, petal and fruit. Plant Cell Physiol. 52 (2011) 851–865. [PMID: 21450689]
4.	Chang, S., Berman, J., Sheng, Y., Wang, Y., Capell, T., Shi, L., Ni, X., Sandmann, G., Christou, P. and Zhu, C. Cloning and functional characterization of the maize (Zea mays L.) carotenoid ε hydroxylase gene. PLoS One 10:e0128758 (2015). [PMID: 26030746]
5.	Reddy, C.S., Lee, S.H., Yoon, J.S., Kim, J.K., Lee, S.W., Hur, M., Koo, S.C., Meilan, J., Lee, W.M., Jang, J.K., Hur, Y., Park, S.U. and Kim, A.YB. Molecular cloning and characterization of carotenoid pathway genes and carotenoid content in Ixeris dentata var. albiflora. Molecules 22 (2017) . [DOI] [PMID: 28858245]

[EC 1.14.14.158 created 2011 as EC 1.14.99.45, transferred 2018 to EC 1.14.14.158]