ExplorEnz: EC 1.14.14.86

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1.14.14.86

Accepted name:

ent-kaurene monooxygenase

Reaction:

ent-kaur-16-ene + 3 [reduced NADPH—hemoprotein reductase] + 3 O₂ = ent-kaur-16-en-19-oate + 3 [oxidized NADPH—hemoprotein reductase] + 4 H₂O (overall reaction)
(1a) ent-kaur-16-ene + [reduced NADPH—hemoprotein reductase] + O₂ = ent-kaur-16-en-19-ol + [oxidized NADPH—hemoprotein reductase] + H₂O
(1b) ent-kaur-16-en-19-ol + [reduced NADPH—hemoprotein reductase] + O₂ = ent-kaur-16-en-19-al + [oxidized NADPH—hemoprotein reductase] + 2 H₂O
(1c) ent-kaur-16-en-19-al + [reduced NADPH—hemoprotein reductase] + O₂ = ent-kaur-16-en-19-oate + [oxidized NADPH—hemoprotein reductase] + H₂O

For diagram of gibberellin A₁₂ biosynthesis, click here

Other name(s):

ent-kaurene oxidase (misleading)

Systematic name:

ent-kaur-16-ene,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (hydroxylating)

Comments:

A cytochrome P-450 (heme thiolate) protein found in plants. Catalyses three successive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 149565-67-3

References:

1.	Ashman, P.J., Mackenzie, A. and Bramley, P.M. Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi. Biochim. Biophys. Acta 1036 (1990) 151–157. [DOI] [PMID: 2223832]
2.	Archer, C., Ashman, P.J., Hedden, P., Bowyer, J.R. and Bramley, P.M. Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima. Biochem. Soc. Trans. 20 (1992) 218. [PMID: 1397591]
3.	Helliwell, C.A., Poole, A., Peacock, W.J. and Dennis, E.S. Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis. Plant Physiol. 119 (1999) 507–510. [PMID: 9952446]

[EC 1.14.14.86 created 2002 as EC 1.14.13.78, transferred 2018 to EC 1.14.14.86]