EC |
4.3.3.5 |
Accepted name: |
4′-demethylrebeccamycin synthase |
Reaction: |
4′-O-demethylrebeccamycin + H2O = dichloro-arcyriaflavin A + β-D-glucose |
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For diagram of rebeccamycin biosynthesis, click here |
Glossary: |
dichloro-arcyriaflavin A = rebeccamycin aglycone |
Other name(s): |
arcyriaflavin A N-glycosyltransferase; RebG |
Systematic name: |
4′-demethylrebeccamycin D-glucose-lyase |
Comments: |
This enzyme catalyses a step in the biosynthesis of rebeccamycin, an indolocarbazole alkaloid produced by the bacterium Lechevalieria aerocolonigenes. The enzyme is a glycosylase, and acts in the reverse direction to that shown. It has a wide substrate range, and was shown to glycosylate several substrates, including the staurosporine aglycone, EJG-III-108A, J-104303, 6-N-methyl-arcyriaflavin C and indolo-[2,3-a]-carbazole [1,2]. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Ohuchi, T., Ikeda-Araki, A., Watanabe-Sakamoto, A., Kojiri, K., Nagashima, M., Okanishi, M. and Suda, H. Cloning and expression of a gene encoding N-glycosyltransferase (ngt) from Saccharothrix aerocolonigenes ATCC39243. J. Antibiot. (Tokyo) 53 (2000) 393–403. [PMID: 10866221] |
2. |
Zhang, C., Albermann, C., Fu, X., Peters, N.R., Chisholm, J.D., Zhang, G., Gilbert, E.J., Wang, P.G., Van Vranken, D.L. and Thorson, J.S. RebG- and RebM-catalyzed indolocarbazole diversification. ChemBioChem 7 (2006) 795–804. [DOI] [PMID: 16575939] |
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[EC 4.3.3.5 created 2010] |
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