| EC |
1.14.13.168 |
| Accepted name: |
indole-3-pyruvate monooxygenase |
| Reaction: |
(indol-3-yl)pyruvate + NADPH + H+ + O2 = (indol-3-yl)acetate + NADP+ + H2O + CO2 |
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For diagram of indoleacetic acid biosynthesis, click here |
| Glossary: |
(indol-3-yl)pyruvate = 3-(1H-indol-3-yl)-2-oxopropanoate, (indol-3-yl)acetate = 2-(1H-indol-3-yl)acetate = indole-3-acetate |
| Other name(s): |
YUC2 (gene name); spi1 (gene name) |
| Systematic name: |
indole-3-pyruvate,NADPH:oxygen oxidoreductase (1-hydroxylating, decarboxylating) |
| Comments: |
This plant enzyme, along with EC 2.6.1.99 L-tryptophan—pyruvate aminotransferase, is responsible for the biosynthesis of the plant hormone indole-3-acetate from L-tryptophan. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Mashiguchi, K., Tanaka, K., Sakai, T., Sugawara, S., Kawaide, H., Natsume, M., Hanada, A., Yaeno, T., Shirasu, K., Yao, H., McSteen, P., Zhao, Y., Hayashi, K., Kamiya, Y. and Kasahara, H. The main auxin biosynthesis pathway in Arabidopsis. Proc. Natl. Acad. Sci. USA 108 (2011) 18512–18517. [DOI] [PMID: 22025724] |
| 2. |
Zhao, Y. Auxin biosynthesis: a simple two-step pathway converts tryptophan to indole-3-acetic acid in plants. Mol. Plant 5 (2012) 334–338. [DOI] [PMID: 22155950] |
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| [EC 1.14.13.168 created 2012] |
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| EC |
1.14.13.235 |
| Accepted name: |
indole-3-acetate monooxygenase |
| Reaction: |
(indol-3-yl)acetate + NADH + H+ + O2 = (2-hydroxy-1H-indol-3-yl)acetate + NAD+ + H2O |
| Glossary: |
(indol-3-yl)acetate =(1H-indol-3-yl)acetate = indole-3-acetate |
| Other name(s): |
iacA (gene name) |
| Systematic name: |
(indol-3-yl)acetate,NADH:oxygen oxidoreductase (2-hydroxylating) |
| Comments: |
The enzyme, characterized from Pseudomonas putida strains, catalyses the first step in a pathway for degradation of the plant hormone indole-3-acetate. When acting on indole, the enzyme forms indoxyl, which reacts spontaneously with oxygen to form the blue dye indigo. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Leveau, J.H. and Lindow, S.E. Utilization of the plant hormone indole-3-acetic acid for growth by Pseudomonas putida strain 1290. Appl. Environ. Microbiol. 71 (2005) 2365–2371. [DOI] [PMID: 15870323] |
| 2. |
Scott, J.C., Greenhut, I.V. and Leveau, J.H. Functional characterization of the bacterial iac genes for degradation of the plant hormone indole-3-acetic acid. J Chem Ecol 39 (2013) 942–951. [DOI] [PMID: 23881445] |
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| [EC 1.14.13.235 created 2017] |
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| EC |
4.1.1.115 |
| Accepted name: |
indoleacetate decarboxylase |
| Reaction: |
(1H-indol-3-yl)acetate = skatole + CO2 |
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For diagram of indoleacetic acid biosynthesis, click here |
| Glossary: |
(1H-indol-3-yl)acetate = indoleacetate
skatole = 3-methyl-1H-indole
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| Other name(s): |
IAD |
| Systematic name: |
(1H-indol-3-yl)acetate carboxy-lyase (skatole-forming) |
| Comments: |
This glycyl radical enzyme has been isolate from a number of bacterial species. Skatole contributes to the characteristic smell of animal faeces. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Liu, D., Wei, Y., Liu, X., Zhou, Y., Jiang, L., Yin, J., Wang, F., Hu, Y., Nanjaraj Urs, A.N., Liu, Y., Ang, E.L., Zhao, S., Zhao, H. and Zhang, Y. Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole. Nat. Commun. 9:4224 (2018). [PMID: 30310076] |
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| [EC 4.1.1.115 created 2019] |
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