The Enzyme Database

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EC 2.4.1.279     
Accepted name: nigerose phosphorylase
Reaction: 3-O-α-D-glucopyranosyl-D-glucopyranose + phosphate = D-glucose + β-D-glucose 1-phosphate
Glossary: 3-O-α-D-glucopyranosyl-D-glucopyranose = nigerose
Other name(s): cphy1874 (gene name)
Systematic name: 3-O-α-D-glucopyranosyl-D-glucopyranose:phosphate β-D-glucosyltransferase
Comments: The enzymes from Clostridium phytofermentans is specific for nigerose, and shows only 0.5% relative activity with kojibiose (cf. EC 2.4.1.230, kojibiose phosphorylase).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Nihira, T., Nakai, H., Chiku, K. and Kitaoka, M. Discovery of nigerose phosphorylase from Clostridium phytofermentans. Appl. Microbiol. Biotechnol. 93 (2012) 1513–1522. [DOI] [PMID: 21808968]
[EC 2.4.1.279 created 2012]
 
 
EC 2.4.1.334     
Accepted name: 1,3-α-oligoglucan phosphorylase
Reaction: [(1→3)-α-D-glucosyl]n + phosphate = [(1→3)-α-D-glucosyl]n-1 + β-D-glucose 1-phosphate
Systematic name: 1,3-α-D-glucan:phosphate β-D-glucosyltransferase
Comments: The enzyme, isolated from the bacterium Clostridium phytofermentans, catalyses a reversible reaction. Substrates for the phosphorolytic reaction are α-1,3-linked oligoglucans with a polymerisation degree of 3 or more. Nigerose (i.e. 3-O-α-D-glucopyranosyl-D-glucopyranose) is not phosphorylyzed but can serve as substrate in the reverse direction (cf. EC 2.4.1.279, nigerose phosphorylase).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Nihira, T., Nishimoto, M., Nakai, H., Ohtsubo, K., and Kitaoka, M. Characterization of two phosphorylases for α-1,3-oligoglucans from Clostridium phytofermentans. J. Appl. Glycosci. 61 (2014) 59–66.
[EC 2.4.1.334 created 2014]
 
 


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