| EC |
1.1.1.421 |
| Accepted name: |
D-apionate oxidoisomerase |
| Reaction: |
D-apionate + NAD+ = 3-oxoisoapionate + NADH + H+ |
| Glossary: |
3-oxoisoapionate = 2,4-dihydroxy-2-(hydroxymethyl)-3-oxobutanoate |
| Other name(s): |
apnO (gene name) |
| Systematic name: |
D-apionate:NAD+ oxidoreductase (isomerizing) |
| Comments: |
The enzyme, characterized from several bacterial species, participates in the degradation of D-apionate. The reaction involves migration of a hydroxymethyl group from position 3 to position 2 and oxidation of the 3-hydroxyl group. Stereospecificity of the product, 3-oxoisoapionate, has not been determined. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
| References: |
| 1. |
Carter, M.S., Zhang, X., Huang, H., Bouvier, J.T., Francisco, B.S., Vetting, M.W., Al-Obaidi, N., Bonanno, J.B., Ghosh, A., Zallot, R.G., Andersen, H.M., Almo, S.C. and Gerlt, J.A. Functional assignment of multiple catabolic pathways for D-apiose. Nat. Chem. Biol. 14 (2018) 696–705. [DOI] [PMID: 29867142] |
|
| [EC 1.1.1.421 created 2019] |
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|
| EC |
2.7.1.231 |
| Accepted name: |
3-oxoisoapionate kinase |
| Reaction: |
ATP + 3-oxoisoapionate = ADP + 3-oxoisoapionate 4-phosphate |
| Glossary: |
3-oxoisoapionate = 2,4-dihydroxy-2-(hydroxymethyl)-3-oxobutanoate |
| Other name(s): |
oiaK (gene name) |
| Systematic name: |
ATP:3-oxoisoapionate 4-phosphotransferase |
| Comments: |
The enzyme, characterized from several bacterial species, participates in the degradation of D-apionate. Stereospecificity of the product, 3-oxoisoapionate 4-phosphate, has not been determined. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Carter, M.S., Zhang, X., Huang, H., Bouvier, J.T., Francisco, B.S., Vetting, M.W., Al-Obaidi, N., Bonanno, J.B., Ghosh, A., Zallot, R.G., Andersen, H.M., Almo, S.C. and Gerlt, J.A. Functional assignment of multiple catabolic pathways for D-apiose. Nat. Chem. Biol. 14 (2018) 696–705. [DOI] [PMID: 29867142] |
|
| [EC 2.7.1.231 created 2020] |
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|
| EC |
3.7.1.28 |
| Accepted name: |
3-oxoisoapionate-4-phosphate transcarboxylase/hydrolase |
| Reaction: |
3-oxoisoapionate 4-phosphate + H2O = glycolate + 3-phospho-D-glycerate |
| Glossary: |
3-oxoisoapionate = 2,4-dihydroxy-2-(hydroxymethyl)-3-oxobutanoate |
| Other name(s): |
oiaT (gene name) |
| Systematic name: |
3-oxoisoapionate-4-phosphate transcarboxylase/glycolylhydrolase (3-phospho-D-glycerate-forming) |
| Comments: |
The enzyme, which belongs to the RuBisCO-like-protein (RLP) superfamily, has been characterized from several bacterial species. It participates in the degradation of D-apionate. The reaction is initiated by decarboxylation to generate a stabilized enediolate intermediate, with the sequestered CO2 carboxylating the adjacent enediolate carbon atom. The resulting 3-ketose-1-phosphate intermediate is hydrolysed, as in the authentic RuBisCO-catalysed reaction, to generate glycolate and 3-phospho-D-glycerate. Stereospecificity of 3-oxoisoapionate 4-phosphate has not been determined. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Carter, M.S., Zhang, X., Huang, H., Bouvier, J.T., Francisco, B.S., Vetting, M.W., Al-Obaidi, N., Bonanno, J.B., Ghosh, A., Zallot, R.G., Andersen, H.M., Almo, S.C. and Gerlt, J.A. Functional assignment of multiple catabolic pathways for D-apiose. Nat. Chem. Biol. 14 (2018) 696–705. [DOI] [PMID: 29867142] |
|
| [EC 3.7.1.28 created 2021] |
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|
| EC |
4.1.1.120 |
| Accepted name: |
3-oxoisoapionate decarboxylase |
| Reaction: |
3-oxoisoapionate = L-erythrulose + CO2 |
| Glossary: |
3-oxoisoapionate = 2,4-dihydroxy-2-(hydroxymethyl)-3-oxobutanoate |
| Other name(s): |
oiaC (gene name) |
| Systematic name: |
3-oxoisoapionate carboxy-lyase |
| Comments: |
The enzyme, characterized from several bacterial species, is involved in the degradation of D-apionate. Stereospecificity of 3-oxoisoapionate has not been determined. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Carter, M.S., Zhang, X., Huang, H., Bouvier, J.T., Francisco, B.S., Vetting, M.W., Al-Obaidi, N., Bonanno, J.B., Ghosh, A., Zallot, R.G., Andersen, H.M., Almo, S.C. and Gerlt, J.A. Functional assignment of multiple catabolic pathways for D-apiose. Nat. Chem. Biol. 14 (2018) 696–705. [DOI] [PMID: 29867142] |
|
| [EC 4.1.1.120 created 2020] |
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|
| EC |
4.1.1.121 |
| Accepted name: |
3-oxoisoapionate-4-phosphate decarboxylase |
| Reaction: |
3-oxoisoapionate 4-phosphate = L-erythrulose 1-phosphate + CO2 |
| Glossary: |
3-oxoisoapionate = 2,4-dihydroxy-2-(hydroxymethyl)-3-oxobutanoate |
| Other name(s): |
oiaX (gene name) |
| Systematic name: |
3-oxoisoapionate 4-phosphate carboxy-lyase |
| Comments: |
The enzyme, characterized from several bacterial species, participates in the degradation of D-apionate. It belongs to the RuBisCO-like-protein (RLP) superfamily. Stereospecificity of 3-oxoisoapionate 4-phosphate has not been determined. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Carter, M.S., Zhang, X., Huang, H., Bouvier, J.T., Francisco, B.S., Vetting, M.W., Al-Obaidi, N., Bonanno, J.B., Ghosh, A., Zallot, R.G., Andersen, H.M., Almo, S.C. and Gerlt, J.A. Functional assignment of multiple catabolic pathways for D-apiose. Nat. Chem. Biol. 14 (2018) 696–705. [DOI] [PMID: 29867142] |
|
| [EC 4.1.1.121 created 2020] |
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