The Enzyme Database

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EC 4.2.99.22     
Accepted name: tuliposide A-converting enzyme
Reaction: 6-tuliposide A = tulipalin A + D-glucose
Glossary: 6-tuliposide A = 6-O-(4-hydroxy-2-methylenebutanoyl)-D-glucose
tulipalin A = 2-methylenebutanolactone
Other name(s): tuliposide-converting enzyme; 6-O-(4′-hydroxy-2′-methylenebutyryl)-D-glucose acyltransferase (lactone-forming); TCA; TCEA
Systematic name: 6-tuliposide A D-glucose-lyase (tulipalin A forming)
Comments: Isolated from the plant Tulipa gesneriana (tulip). The reaction is an intramolecular transesterification producing the lactone. The enzyme also has a weak activity with 6-tuliposide B and 6-O-benzoyl-D-glucose.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Kato, Y., Shoji, K., Ubukata, M., Shigetomi, K., Sato, Y., Nakajima, N. and Ogita, S. Purification and characterization of a tuliposide-converting enzyme from bulbs of Tulipa gesneriana. Biosci. Biotechnol. Biochem. 73 (2009) 1895–1897. [DOI] [PMID: 19661715]
2.  Nomura, T., Ogita, S. and Kato, Y. A novel lactone-forming carboxylesterase: molecular identification of a tuliposide A-converting enzyme in tulip. Plant Physiol. 159 (2012) 565–578. [DOI] [PMID: 22474185]
[EC 4.2.99.22 created 2013]
 
 
EC 4.2.99.23     
Accepted name: tuliposide B-converting enzyme
Reaction: 6-tuliposide B = tulipalin B + D-glucose
Glossary: 6-tuliposide B = 6-O-[(3S)-3,4-dihydroxy-2-methylenebutanoyl]-D-glucose
tulipalin B = (4S)-4-hydroxy-3-methylideneoxolan-2-one
Systematic name: 6-tuliposide B D-glucose-lyase (tulipalin B-forming)
Comments: The enzyme, characterized from pollen of the plant Tulipa gesneriana (tulip), catalyses the intramolecular transesterification of 6-tuliposide B to form the antibiotic aglycon tulipalin B as a sole product. It does not catalyse the hydrolysis of 6-tuliposide B to form a hydroxy acid. The enzyme has marginal activity with 6-tuliposide A. cf. EC 4.2.99.22, tuliposide A-converting enzyme.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Nomura, T., Murase, T., Ogita, S. and Kato, Y. Molecular identification of tuliposide B-converting enzyme: a lactone-forming carboxylesterase from the pollen of tulip. Plant J. 83 (2015) 252–262. [DOI] [PMID: 25997073]
[EC 4.2.99.23 created 2016]
 
 


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