The Enzyme Database

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EC 1.3.1.97     
Accepted name: botryococcene synthase
Reaction: C30 botryococcene + NADP+ + diphosphate = presqualene diphosphate + NADPH + H+
For diagram of botryococcus braunii BOT22 squalene and botrycoccene biosynthesis, click here
Glossary: C30 botryococcene = (10S,13R)-10-ethenyl-2,6,10,13,17,21-hexamethyldocosa-2,5,11,16,20-pentaene
Other name(s): SSL-3 (gene name)
Systematic name: C30 botryococcene:NADP+ oxidoreductase
Comments: Isolated from the green alga Botryococcus braunii BOT22. Acts in the reverse direction. Involved in the production of botryococcenes, which are triterpenoid hydrocarbons of isoprenoid origin produced in large amount by this alga.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Niehaus, T.D., Okada, S., Devarenne, T.P., Watt, D.S., Sviripa, V. and Chappell, J. Identification of unique mechanisms for triterpene biosynthesis in Botryococcus braunii. Proc. Natl. Acad. Sci. USA 108 (2011) 12260–12265. [DOI] [PMID: 21746901]
[EC 1.3.1.97 created 2012]
 
 
EC 2.1.1.263     
Accepted name: botryococcene C-methyltransferase
Reaction: 2 S-adenosyl-L-methionine + C30 botryococcene = 2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene (overall reaction)
(1a) S-adenosyl-L-methionine + C30 botryococcene = S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene
(1b) S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene = S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
(2a) S-adenosyl-L-methionine + C30 botryococcene = S-adenosyl-L-homocysteine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene
(2b) S-adenosyl-L-methionine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene = S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene
For diagram of botryococcus braunii BOT22 squalene and botrycoccene biosynthesis, click here
Glossary: C30 botryococcene = (10S,13R)-10-ethenyl-2,6,10,13,17,21-hexamethyldocosa-2,5,11,16,20-pentaene
3-methyl-1,2-didehydro-2,3-dihydrobotryococcene = showacene
20-methyl-21,22-didehydro-20,21-dihydrobotryococcene = isoshowacene
Other name(s): TMT-3
Systematic name: S-adenosyl-L-methionine:botryococcene C-methyltransferase
Comments: Isolated from the green alga Botryococcus braunii BOT22. Shows a very weak activity with squalene.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Niehaus, T.D., Kinison, S., Okada, S., Yeo, Y.S., Bell, S.A., Cui, P., Devarenne, T.P. and Chappell, J. Functional identification of triterpene methyltransferases from Botryococcus braunii race B. J. Biol. Chem. 287 (2012) 8163–8173. [DOI] [PMID: 22241476]
[EC 2.1.1.263 created 2012]
 
 


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