The Enzyme Database

Your query returned 3 entries.    printer_iconPrintable version

EC 3.5.4.43     
Accepted name: hydroxydechloroatrazine ethylaminohydrolase
Reaction: hydroxyatrazine + H2O = N-isopropylammelide + ethylamine
For diagram of atrazine catabolism, click here
Glossary: hydroxyatrazine = 4-(ethylamino)-6-(isopropylamino)-1,3,5-triazin-2-ol
N-isopropylammelide = 6-(isopropylamino)-1,3,5-triazine-2,4-diol
Other name(s): atzB (gene name); 2,4-dihydroxy-6-(isopropylamino)-1,3,5-triazine ethylaminohydrolase
Systematic name: hydroxyatrazine ethylaminohydrolase
Comments: Contains Zn2+. This bacterial enzyme is involved in degradation of the herbicide atrazine. The enzyme has a broad substrate range, and requires a monohydroxylated s-triazine ring with a minimum of one primary or secondary amine substituent and either a chloride or amine leaving group. It catalyses both deamination and dechlorination reactions.
Links to other databases: BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc
References:
1.  Boundy-Mills, K.L., de Souza, M.L., Mandelbaum, R.T., Wackett, L.P. and Sadowsky, M.J. The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathway. Appl. Environ. Microbiol. 63 (1997) 916–923. [PMID: 9055410]
2.  Seffernick, J.L., Aleem, A., Osborne, J.P., Johnson, G., Sadowsky, M.J. and Wackett, L.P. Hydroxyatrazine N-ethylaminohydrolase (AtzB): an amidohydrolase superfamily enzyme catalyzing deamination and dechlorination. J. Bacteriol. 189 (2007) 6989–6997. [DOI] [PMID: 17660279]
[EC 3.5.4.43 created 2000 as EC 3.5.99.3, transferred 2016 to EC 3.5.4.43]
 
 
EC 3.5.99.3      
Transferred entry: hydroxydechloroatrazine ethylaminohydrolase. Now EC 3.5.4.43, hydroxydechloroatrazine ethylaminohydrolase
[EC 3.5.99.3 created 2000, deleted 2016]
 
 
EC 3.8.1.8     
Accepted name: atrazine chlorohydrolase
Reaction: atrazine + H2O = hydroxyatrazine + chloride
For diagram of atrazine catabolism, click here
Glossary: atrazine = 6-chloro-N-ethyl-N′-isopropyl-1,3,5-triazine-2,4-diamine
hydroxyatrazine = 4-(ethylamino)-6-(isopropylamino)-1,3,5-triazin-2-ol
Other name(s): AtzA
Systematic name: atrazine chlorohydrolase
Comments: Involved in the degradation of the herbicide atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, in bacteria.
Links to other databases: BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 168680-16-8
References:
1.  de Souza, M.L., Wackett, L.P., Boundy-Mills, K.L., Mandelbaum, R.T. and Sadowsky, M.J. Cloning, characterization, and expression of a gene region from Pseudomonas sp. strain ADP involved in the dechlorination of atrazine. Appl. Environ. Microbiol. 61 (1995) 3373–3378. [PMID: 7574646]
2.  de Souza, M.L., Sadowsky, M.J. and Wackett, L.P. Atrazine chlorohydrolase from Pseudomonas sp. strain ADP: gene sequence, enzyme purification, and protein characterization. J. Bacteriol. 178 (1996) 4894–4900. [DOI] [PMID: 8759853]
[EC 3.8.1.8 created 2000, modified 2011]
 
 


Data © 2001–2024 IUBMB
Web site © 2005–2024 Andrew McDonald