| EC |
1.8.1.15 |
| Accepted name: |
mycothione reductase |
| Reaction: |
2 mycothiol + NAD(P)+ = mycothione + NAD(P)H + H+ |
| Glossary: |
mycothiol = 1-O-[2-(N2-acetyl-L-cysteinamido)-2-deoxy--D-glucopyranosyl]-1D-myo-inositol
mycothione = oxidized (disulfide) form of mycothiol |
| Other name(s): |
mycothiol-disulfide reductase |
| Systematic name: |
mycothiol:NAD(P)+ oxidoreductase |
| Comments: |
Contains FAD. No activity with glutathione, trypanothione or coenzyme A as substrate. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 252212-92-3 |
| References: |
| 1. |
Patel, M.P. and Blanchard, J.S. Expression, purification, and characterization of Mycobacterium tuberculosis mycothione reductase. Biochemistry 38 (1999) 11827–11833. [DOI] [PMID: 10512639] |
| 2. |
Patel, M.P. and Blanchard, J.S. Mycobacterium tuberculosis mycothione reductase: pH dependence of the kinetic parameters and kinetic isotope effects. Biochemistry 40 (2001) 5119–5126. [DOI] [PMID: 11318633] |
|
| [EC 1.8.1.15 created 2002] |
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|
| EC |
1.20.4.3 |
| Accepted name: |
mycoredoxin |
| Reaction: |
arseno-mycothiol + mycoredoxin = arsenite + mycothiol-mycoredoxin disulfide |
| Glossary: |
mycothiol = 1-O-[2-(N2-acetyl-L-cysteinamido)-2-deoxy-α-D-glucopyranosyl]-1D-myo-inositol |
| Other name(s): |
Mrx1; MrxI |
| Systematic name: |
arseno-mycothiol:mycoredoxin oxidoreductase |
| Comments: |
Reduction of arsenate is part of a defense mechanism of the cell against toxic arsenate. The substrate arseno-mycothiol is formed by EC 2.8.4.2 (arsenate:mycothiol transferase). A second mycothiol recycles mycoredoxin and forms mycothione. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
| References: |
| 1. |
Ordonez, E., Van Belle, K., Roos, G., De Galan, S., Letek, M., Gil, J.A., Wyns, L., Mateos, L.M. and Messens, J. Arsenate reductase, mycothiol, and mycoredoxin concert thiol/disulfide exchange. J. Biol. Chem. 284 (2009) 15107–15116. [DOI] [PMID: 19286650] |
|
| [EC 1.20.4.3 created 2010] |
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|
| EC |
2.8.4.2 |
| Accepted name: |
arsenate-mycothiol transferase |
| Reaction: |
arsenate + mycothiol = arseno-mycothiol + H2O |
| Glossary: |
mycothiol = 1-O-[2-(N2-acetyl-L-cysteinamido)-2-deoxy--D-glucopyranosyl]-1D-myo-inositol |
| Other name(s): |
ArsC1; ArsC2; mycothiol:arsenate transferase |
| Systematic name: |
mycothiol:arsenate S-arsenotransferase |
| Comments: |
Reduction of arsenate is part of a defence mechanism of the cell against toxic arsenate. The product arseno-mycothiol is reduced by EC 1.20.4.3 (mycoredoxin) to arsenite and mycothiol-mycoredoxin disulfide. Finally, a second mycothiol recycles mycoredoxin and forms mycothione. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
| References: |
| 1. |
Ordonez, E., Van Belle, K., Roos, G., De Galan, S., Letek, M., Gil, J.A., Wyns, L., Mateos, L.M. and Messens, J. Arsenate reductase, mycothiol, and mycoredoxin concert thiol/disulfide exchange. J. Biol. Chem. 284 (2009) 15107–15116. [DOI] [PMID: 19286650] |
|
| [EC 2.8.4.2 created 2010] |
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