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Your query returned 8 entries. Printable version
EC | 2.1.3.13 | ||||||
Deleted entry: | ATP carbamoyltransferase. The enzyme has been replaced by EC 6.1.2.2, nebramycin 5′ synthase. | ||||||
EC | 2.1.3.14 | ||||||
Deleted entry: | tobramycin carbamoyltransferase. The enzyme has been replaced by EC 6.1.2.2, nebramycin 5′ synthase | ||||||
EC | 2.3.1.59 | ||||||
Accepted name: | gentamicin 2′-N-acetyltransferase | ||||||
Reaction: | acetyl-CoA + gentamicin C1a = CoA + N2′-acetylgentamicin C1a | ||||||
Glossary: | kanamycin | ||||||
Other name(s): | gentamycin acetyltransferase II; gentamycin 2′-N-acetyltransferase; acetyl-CoA:gentamycin-C1a N2′-acetyltransferase | ||||||
Systematic name: | acetyl-CoA:gentamicin-C1a N2′-acetyltransferase | ||||||
Comments: | The antibiotics gentamicin A, sisomicin, tobramycin, paromomycin, neomycin B, kanamycin B and kanamycin C can also act as acceptors. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 50864-40-9 | ||||||
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EC | 2.3.1.81 | ||||||
Accepted name: | aminoglycoside 3-N-acetyltransferase | ||||||
Reaction: | acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N3-acetyl-2-deoxystreptamine antibiotic | ||||||
For diagram of neamine and ribostamycin biosynthesis, click here | |||||||
Glossary: | kanamycin | ||||||
Other name(s): | 3-aminoglycoside acetyltransferase; 3-N-aminoglycoside acetyltransferase; aminoglycoside N3-acetyltransferase; acetyl-CoA:2-deoxystreptamine-antibiotic N3′-acetyltransferase (incorrect); aminoglycoside N3′-acetyltransferase (incorrect) | ||||||
Systematic name: | acetyl-CoA:2-deoxystreptamine-antibiotic N3-acetyltransferase | ||||||
Comments: | Different from EC 2.3.1.60 gentamicin 3-N-acetyltransferase. A wide range of antibiotics containing the 2-deoxystreptamine ring can act as acceptors, including gentamicin, kanamycin, tobramycin, neomycin and apramycin. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 60120-42-5 | ||||||
References: |
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EC | 2.3.1.82 | ||||||
Accepted name: | aminoglycoside 6′-N-acetyltransferase | ||||||
Reaction: | acetyl-CoA + kanamycin-B = CoA + N6′-acetylkanamycin-B | ||||||
Glossary: | kanamycin | ||||||
Other name(s): | aminoglycoside N6′-acetyltransferase; aminoglycoside-6′-acetyltransferase; aminoglycoside-6-N-acetyltransferase; kanamycin acetyltransferase | ||||||
Systematic name: | acetyl-CoA:kanamycin-B N6′-acetyltransferase | ||||||
Comments: | The antibiotics kanamycin A, kanamycin B, neomycin, gentamicin C1a, gentamicin C2 and sisomicin are substrates. The antibiotic tobramycin, but not paromomycin, can also act as acceptor. The 6-amino group of the purpurosamine ring is acetylated. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 56467-65-3 | ||||||
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EC | 2.7.1.190 | ||||||
Accepted name: | aminoglycoside 2′′-phosphotransferase | ||||||
Reaction: | GTP + gentamicin = GDP + gentamicin 2′′-phosphate | ||||||
Other name(s): | aphD (gene name); APH(2′′); aminoglycoside (2′′) kinase; gentamicin kinase (ambiguous); gentamicin phosphotransferase (ambiguous) | ||||||
Systematic name: | GTP:gentamicin 2′′-O-phosphotransferase | ||||||
Comments: | Requires Mg2+. This bacterial enzyme phosphorylates many 4,6-disubstituted aminoglycoside antibiotics that have a hydroxyl group at position 2′′, including kanamycin A, kanamycin B, tobramycin, dibekacin, arbekacin, amikacin, gentamicin C, sisomicin and netilmicin. In most, but not all, cases the phosphorylation confers resistance against the antibiotic. Some forms of the enzyme use ATP as a phosphate donor in appreciable amount. The enzyme is often found as a bifunctional enzyme that also catalyses 6′-aminoglycoside N-acetyltransferase activity. The bifunctional enzyme is the most clinically important aminoglycoside-modifying enzyme in Gram-positive bacteria, responsible for high-level resistance in both Enterococci and Staphylococci. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB | ||||||
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EC | 2.7.7.46 | ||||||
Accepted name: | gentamicin 2′′-nucleotidyltransferase | ||||||
Reaction: | nucleoside triphosphate + gentamicin = diphosphate + 2′′-nucleotidylgentamicin | ||||||
Other name(s): | gentamicin 2′′-adenylyltransferase; aminoglycoside adenylyltransferase; gentamycin 2′′-nucleotidyltransferase | ||||||
Systematic name: | NTP:gentamicin 2′′-nucleotidyltransferase | ||||||
Comments: | ATP, dATP, CTP, ITP and GTP can act as donors; kanamycin, tobramycin and sisomicin can also act as acceptors. The nucleotidyl residue is transferred to the 2-hydroxy of the 3-amino-3-deoxy-D-glucose moiety in the antibiotic. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 62213-33-6 | ||||||
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EC | 6.1.2.2 | ||||||
Accepted name: | nebramycin 5′ synthase | ||||||
Reaction: | (1) tobramycin + carbamoyl phosphate + ATP + H2O = nebramycin 5′ + AMP + diphosphate + phosphate (overall reaction) (1a) carbamoyl phosphate + ATP + H2O = diphosphate + O-carbamoyladenylate + phosphate (1b) O-carbamoyladenylate + tobramycin = AMP + nebramycin 5′ (2) kanamycin A + carbamoyl phosphate + ATP + H2O = 6′′-O-carbamoylkanamycin A + AMP + diphosphate + phosphate (overall reaction) (2a) carbamoyl phosphate + ATP + H2O = diphosphate + O-carbamoyladenylate + phosphate (2b) O-carbamoyladenylate + kanamycin A = AMP + 6′′-O-carbamoylkanamycin A |
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For diagram of kanamycin A biosynthesis, click here | |||||||
Glossary: | tobramycin = (1S,2S,3R,4S,6R)-4,6-diamino-3-(2,6-diamino-2,3,6-trideoxy-α-D-ribo-hexopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy-α-D-glucopyranoside nebramycin 5′ = (1S,2S,3R,4S,6R)-4,6-diamino-3-[(2,6-diamino-2,3,6-trideoxy-α-D-ribo-hexopyranosyl)oxy]-2-hydroxycyclohexyl 3-amino-6-O-carbamoyl-3-deoxy-α-D-glucopyranoside kanamycin A = (1S,2R,3R,4S,6R)-4,6-diamino-3-(6-amino-6-deoxy--D-glucopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy--D-glucopyranoside 6′′-O-carbamoylkanamycin A = (1S,2R,3R,4S,6R)-4,6-diamino-3-[(6-amino-6-deoxy-α-D-glucopyranosyl)oxy]-2-hydroxycyclohexyl 3-amino-6-O-carbamoyl-3-deoxy-α-D-glucopyranoside |
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Other name(s): | tobramycin carbamoyltransferase; TobZ | ||||||
Systematic name: | tobramycin:carbamoyl phosphate ligase (AMP,phosphate-forming) | ||||||
Comments: | Requires Fe(III). The enzyme from the bacterium Streptoalloteichus tenebrarius catalyses the activation of carbamoyl phosphate to O-carbamoyladenylate and the subsequent carbamoylation of kanamycin and tobramycin. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB | ||||||
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