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Your query returned 6 entries. Printable version
EC | 2.1.1.98 | ||||||||
Accepted name: | diphthine synthase | ||||||||
Reaction: | 3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] = 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] (overall reaction) (1a) S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] (1b) S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] (1c) S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] |
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For diagram of diphthamide biosynthesis, click here | |||||||||
Glossary: | diphthine = 2-[(3S)-3-carboxy-3-(trimethylamino)propyl]-L-histidine | ||||||||
Other name(s): | S-adenosyl-L-methionine:elongation factor 2 methyltransferase (ambiguous); diphthine methyltransferase (ambiguous); S-adenosyl-L-methionine:2-(3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] methyltransferase; Dph5 (ambiguous) | ||||||||
Systematic name: | S-adenosyl-L-methionine:2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] methyltransferase (diphthine-[translation elongation factor 2]-forming) | ||||||||
Comments: | This archaeal enzyme produces the trimethylated product diphthine, which is converted into diphthamide by EC 6.3.1.14, diphthine—ammonia ligase. Different from the eukaryotic enzyme, which produces diphthine methyl ester (cf. EC 2.1.1.314). In the archaeon Pyrococcus horikoshii the enzyme acts on His600 of elongation factor 2. | ||||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 114514-25-9 | ||||||||
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EC | 2.1.1.314 | ||||||||
Accepted name: | diphthine methyl ester synthase | ||||||||
Reaction: | 4 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] = 4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2] | ||||||||
For diagram of diphthamide biosynthesis, click here | |||||||||
Glossary: | diphthine methyl ester = 2-[(3S)-4-methoxy-4-oxo-3-(trimethylammonio)butyl]-L-histidine | ||||||||
Other name(s): | S-adenosyl-L-methionine:elongation factor 2 methyltransferase (ambiguous); diphthine methyltransferase (ambiguous); Dph5 (ambiguous) | ||||||||
Systematic name: | S-adenosyl-L-methionine:2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] methyltransferase (diphthine methyl ester-[translation elongation factor 2]-forming) | ||||||||
Comments: | This eukaryotic enzyme is part of the biosynthetic pathway of diphthamide. Different from the archaeal enzyme, which performs only 3 methylations, producing diphthine (cf. EC 2.1.1.98). The relevant histidine of elongation factor 2 is His715 in mammals and His699 in yeast. The order of the 4 methylations is not known. | ||||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB | ||||||||
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EC | 2.4.2.36 | ||||||||
Accepted name: | NAD+—diphthamide ADP-ribosyltransferase | ||||||||
Reaction: | NAD+ + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2] | ||||||||
For diagram of diphthamide biosynthesis, click here | |||||||||
Glossary: | diphthamide = 2-[4-amino-4-oxo-3-(trimethylammonio)butyl]-L-histidine | ||||||||
Other name(s): | ADP-ribosyltransferase; mono(ADPribosyl)transferase; NAD—diphthamide ADP-ribosyltransferase; NAD+:peptide-diphthamide N-(ADP-D-ribosyl)transferase | ||||||||
Systematic name: | NAD+:diphthamide-[translation elongation factor 2] N-(ADP-D-ribosyl)transferase | ||||||||
Comments: | Diphtheria toxin and some other bacterial toxins catalyse this reaction, which inactivates translation elongation factor 2 (EF2). The acceptor is diphthamide, a unique modification of a histidine residue in the elongation factor found in archaebacteria and all eukaryotes, but not in eubacteria. cf. EC 2.4.2.31 NAD(P)+—protein-arginine ADP-ribosyltransferase. The relevant histidine of EF2 is His715 in mammals, His699 in yeast and His600 in Pyrococcus horikoshii. | ||||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 52933-21-8 | ||||||||
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EC | 2.5.1.108 | ||||||||
Accepted name: | 2-(3-amino-3-carboxypropyl)histidine synthase | ||||||||
Reaction: | S-adenosyl-L-methionine + L-histidine-[translation elongation factor 2] = S-methyl-5′-thioadenosine + 2-[(3S)-3-amino-3-carboxypropyl]-L-histidine-[translation elongation factor 2] | ||||||||
For diagram of diphthamide biosynthesis, click here | |||||||||
Other name(s): | Dph2 | ||||||||
Systematic name: | S-adenosyl-L-methionine:L-histidine-[translation elongation factor 2] 2-[(3S)-3-amino-3-carboxypropyl]transferase | ||||||||
Comments: | A [4Fe-4S] enzyme that modifies a histidine residue of the translation elongation factor 2 (EF2) via a 3-amino-3-carboxypropyl radical. The enzyme is present in archae and eukaryotes but not in eubacteria. The enzyme is a member of the ’AdoMet radical’ (radical SAM) family and generates the 3-amino-3-carboxypropyl radical by an uncanonical clevage of S-adenosyl-L-methionine. The relevant histidine of EF2 is His715 in mammals, His699 in yeast and His600 in Pyrococcus horikoshii. Part of diphthamide biosynthesis. | ||||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB | ||||||||
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EC | 3.1.1.97 | ||||||||
Accepted name: | methylated diphthine methylhydrolase | ||||||||
Reaction: | diphthine methyl ester-[translation elongation factor 2] + H2O = diphthine-[translation elongation factor 2] + methanol | ||||||||
For diagram of diphthamide biosynthesis, click here | |||||||||
Glossary: | diphthine methyl ester = 2-[(3S)-3-carboxy methyl ester-3-(trimethylammonio)propyl]-L-histidine diphthine = 2-[(3S)-3-carboxy-3-(trimethylammonio)propyl]-L-histidine |
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Other name(s): | Dph7; diphthine methylesterase (incorrect) | ||||||||
Systematic name: | diphthine methyl ester acylhydrolase | ||||||||
Comments: | The protein is only present in eukaryotes. | ||||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc | ||||||||
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EC | 6.3.1.14 | ||||||||
Accepted name: | diphthine—ammonia ligase | ||||||||
Reaction: | ATP + diphthine-[translation elongation factor 2] + NH3 = AMP + diphosphate + diphthamide-[translation elongation factor 2] | ||||||||
For diagram of diphthamide biosynthesis, click here | |||||||||
Glossary: | translation elongation factor 2 = EF2 = eEF2 diphthine = 2-[(3S)-3-carboxy-3-(trimethylammonio)propyl]-L-histidine diphthamide =2-[(3S)-3-carbamoyl-3-(trimethylammonio)propyl]-L-histidine |
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Other name(s): | diphthamide synthase; diphthamide synthetase; DPH6 (gene name); ATPBD4 (gene name); diphthine:ammonia ligase (AMP-forming) | ||||||||
Systematic name: | diphthine-[translation elongation factor 2]:ammonia ligase (AMP-forming) | ||||||||
Comments: | This amidase catalyses the last step in the conversion of an L-histidine residue in the translation elongation factor EF2 to diphthamide. This factor is found in all archaea and eukaryota, but not in eubacteria, and is the target of bacterial toxins such as the diphtheria toxin and the Pseudomonas exotoxin A (see EC 2.4.2.36, NAD+—diphthamide ADP-ribosyltransferase). The substrate of the enzyme, diphthine, is produced by EC 2.1.1.98, diphthine synthase. | ||||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 114514-33-9 | ||||||||
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