EC 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
This subclass contains enzymes that act on two hydrogen-donors, and oxygen is incorporated into one or both of them. Sub-subclasses are based on the second donor and the number of oxygen atoms that are incorporated into one or both donors: 2-oxoglutarate is one donor and one atom of oxygen is incorporated into each donor (EC 1.14.11), NADH or NADPH is one donor, and two atoms of oxygen are incorporated into the other donor (EC 1.14.12), NADH or NADPH is one donor, but only one atom of oxygen is incorporated into the other donor (EC 1.14.13). In sub-subclasses EC 1.14.14-1.14.18, one atom of oxygen is incorporated into one donor, the other donor being a reduced flavin or flavoprotein (EC 1.14.14), a reduced iron-sulfur protein (EC 1.14.15), a reduced pteridine (EC 1.14.16), reduced ascorbate (EC 1.14.17), or some other compound (EC 1.14.18). Sub-subclass EC 1.14.19 differs from others in subclass EC 1.14 in that hydrogen atoms removed from the two donors are combined with O2 to form two molecules of water. Sub-subclass EC 1.14.20 has 2-oxoglutarate as one donor, and the other is dehydrogenated. Sub-subclass EC 1.14.21 has NADH or NADPH as one donor, and the other is dehydrogenated. Sub-subclass EC 1.14.99 is for cases where information about the second donor is incomplete.