ExplorEnz: EC 1.1.1.267

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1.1.1.267

Accepted name:

1-deoxy-D-xylulose-5-phosphate reductoisomerase

Reaction:

2-C-methyl-D-erythritol 4-phosphate + NADP⁺ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H⁺

For diagram of non-mevalonate terpenoid biosynthesis, click here

Other name(s):

DXP-reductoisomerase; 1-deoxy-D-xylulose-5-phosphate isomeroreductase; 2-C-methyl-D-erythritol 4-phosphate (MEP) synthase

Systematic name:

2-C-methyl-D-erythritol-4-phosphate:NADP⁺ oxidoreductase (isomerizing)

Comments:

The enzyme requires Mn²⁺, Co²⁺ or Mg²⁺ for activity, with the first being most effective. The enzyme from several eubacteria, including Escherichia coli, forms part of an alternative nonmevalonate pathway for terpenoid biosynthesis (for diagram, click here). The mechanism has been shown to be a retroaldol/aldol reaction [2].

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 210756-42-6

References:

1.	Takahashi, S., Kuzuyama, T., Watanabe, H. and Seto, H. A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis. Proc. Natl. Acad. Sci. USA 95 (1998) 9879–9884. [DOI] [PMID: 9707569]
2.	Munos, J.W., Pu, X., Mansoorabadi, S.O., Kim, H.J. and Liu, H.W. A secondary kinetic isotope effect study of the 1-deoxy-D-xylulose-5-phosphate reductoisomerase-catalyzed reaction: evidence for a retroaldol-aldol rearrangement. J. Am. Chem. Soc. 131 (2009) 2048–2049. [DOI] [PMID: 19159292]

[EC 1.1.1.267 created 2001]