ExplorEnz: EC 1.1.1.356

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1.1.1.356

Accepted name:

GDP-L-colitose synthase

Reaction:

GDP-β-L-colitose + NAD(P)⁺ = GDP-4-dehydro-3,6-dideoxy-α-D-mannose + NAD(P)H + H⁺

For diagram of GDP-colitose biosynthesis, click here

Glossary:

L-colitose = 3,6-dideoxy-L-xylo-hexopyranose
GDP-4-dehydro-3,6-dideoxy-α-D-mannose = GDP-3,6-dideoxy-α-D-threo-hexopyranos-4-ulose

Other name(s):

ColC

Systematic name:

GDP-β-L-colitose:NAD(P)⁺ 4-oxidoreductase (5-epimerizing)

Comments:

The enzyme is involved in biosynthesis of L-colitose, a 3,6-dideoxyhexose found in the O-antigen of Gram-negative lipopolysaccharides, where it catalyses the reaction in the reverse direction. The enzyme also performs the NAD(P)H-dependent epimerisation at C-5 of the sugar. The enzyme from Yersinia pseudotuberculosis is Si-specific with respect to NAD(P)H [1].

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Alam, J., Beyer, N. and Liu, H.W. Biosynthesis of colitose: expression, purification, and mechanistic characterization of GDP-4-keto-6-deoxy-D-mannose-3-dehydrase (ColD) and GDP-L-colitose synthase (ColC). Biochemistry 43 (2004) 16450–16460. [DOI] [PMID: 15610039]

[EC 1.1.1.356 created 2013]