EC |
1.1.1.382 |
Accepted name: |
ketol-acid reductoisomerase (NAD+) |
Reaction: |
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ |
Glossary: |
(2S)-2-hydroxy-2-methyl-3-oxobutanoate = (2S)-2-acetolactate |
Systematic name: |
(2R)-2,3-dihydroxy-3-methylbutanoate:NAD+ oxidoreductase (isomerizing) |
Comments: |
The enzyme, characterized from the bacteria Thermacetogenium phaeum and Desulfococcus oleovorans and from the archaeon Archaeoglobus fulgidus, is specific for NADH [cf. EC 1.1.1.86, ketol-acid reductoisomerase (NADP+) and EC 1.1.1.383, ketol-acid reductoisomerase [NAD(P)+]]. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Brinkmann-Chen, S., Cahn, J.K. and Arnold, F.H. Uncovering rare NADH-preferring ketol-acid reductoisomerases. Metab. Eng. 26C (2014) 17–22. [DOI] [PMID: 25172159] |
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[EC 1.1.1.382 created 2015] |
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