| EC |
1.1.3.8 |
| Accepted name: |
L-gulonolactone oxidase |
| Reaction: |
L-gulono-1,4-lactone + O2 = L-ascorbate + H2O2 (overall reaction)
(1a) L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
(1b) L-xylo-hex-2-ulono-1,4-lactone = L-ascorbate (spontaneous) |
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For diagram of mammalian ascorbic acid biosynthesis, click here |
| Other name(s): |
L-gulono-γ-lactone: O2 oxidoreductase; L-gulono-γ-lactone oxidase; L-gulono-γ-lactone:oxidoreductase; GLO |
| Systematic name: |
L-gulono-1,4-lactone:oxygen 3-oxidoreductase |
| Comments: |
A microsomal flavoprotein (FAD). The product spontaneously isomerizes to L-ascorbate. While most higher animals can synthesize asborbic acid, primates and guinea pigs cannot [3]. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 9028-78-8 |
| References: |
| 1. |
Isherwood, F.A., Mapson, L.W. and Chen, Y.T. Synthesis of L-ascorbic acid in rat liver homogenates. Conversion of L-gulono- and L-galactono-γ-lactone and the respective acids into L-ascorbic acid. Biochem. J. 76 (1960) 157–171. [PMID: 14405898] |
| 2. |
Kiuchi, K., Noshikimi, M. and Yagi, K. Purification and characterization of L-gulonolactone oxidase from chicken kidney microsomes. Biochemistry 21 (1982) 5076–5082. [PMID: 7138847] |
| 3. |
Nishikimi, M., Fukuyama, R., Minoshima, S., Shimizu, N. and Yagi, K. Cloning and chromosomal mapping of the human nonfunctional gene for L-gulono-γ-lactone oxidase, the enzyme for L-ascorbic acid biosynthesis missing in man. J. Biol. Chem. 269 (1994) 13685–13688. [PMID: 8175804] |
| 4. |
Chatterjee, I.B., Chatterjee, G.C., Ghosh, N.C. and Guha, B.C. Identification of 2-keto-L-gulonolactone as an intermediate in the biosynthesis of L-ascorbic acid. Naturwissenschaften 46 (1959) 475. |
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| [EC 1.1.3.8 created 1965, modified 2001, modified 2006] |
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