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Your query returned 1 entry. 
Printable version
| EC | 1.1.99.31 | ||||||
| Accepted name: | (S)-mandelate dehydrogenase | ||||||
| Reaction: | (S)-mandelate + acceptor = phenylglyoxylate + reduced acceptor | ||||||
| For diagram of reaction, click here | |||||||
| Glossary: | (S)-mandelate = (S)-2-hydroxy-2-phenylacetate phenylglyoxylate = benzoylformate = 2-oxo-2-phenylacetate |
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| Other name(s): | MDH (ambiguous) | ||||||
| Systematic name: | (S)-mandelate:acceptor 2-oxidoreductase | ||||||
| Comments: | This enzyme is a member of the FMN-dependent α-hydroxy-acid oxidase/dehydrogenase family [1]. While all enzymes of this family oxidize the (S)-enantiomer of an α-hydroxy acid to an α-oxo acid, the ultimate oxidant (oxygen, intramolecular heme or some other acceptor) depends on the particular enzyme. This enzyme transfers the electron pair from FMNH2 to a component of the electron transport chain, most probably ubiquinone [1,2]. It is part of a metabolic pathway in Pseudomonads that allows these organisms to utilize mandelic acid, derivatized from the common soil metabolite amygdalin, as the sole source of carbon and energy [2]. The enzyme has a large active-site pocket and preferentially binds substrates with longer sidechains, e.g. 2-hydroxyoctanoate rather than 2-hydroxybutyrate [1]. It also prefers substrates that, like (S)-mandelate, have β unsaturation, e.g. (indol-3-yl)glycolate compared with (indol-3-yl)lactate [1]. Esters of mandelate, such as methyl (S)-mandelate, are also substrates [3]. | ||||||
| Links to other databases: | BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 9067-95-2 | ||||||
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