| EC |
1.14.11.20 |
| Accepted name: |
deacetoxyvindoline 4-hydroxylase |
| Reaction: |
deacetoxyvindoline + 2-oxoglutarate + O2 = deacetylvindoline + succinate + CO2 |
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For diagram of vindoline biosynthesis, click here |
| Other name(s): |
desacetoxyvindoline 4-hydroxylase; desacetyoxyvindoline-17-hydroxylase; D17H; desacetoxyvindoline,2-oxoglutarate:oxygen oxidoreductase (4β-hydroxylating) |
| Systematic name: |
deacetoxyvindoline,2-oxoglutarate:oxygen oxidoreductase (4β-hydroxylating) |
| Comments: |
Requires Fe2+ and ascorbate. Also acts on 3-hydroxy-16-methoxy-2,3-dihydrotabersonine and to a lesser extent on 16-methoxy-2,3-dihydrotabersonine. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 132084-83-4 |
| References: |
| 1. |
De Carolis, E., Chan, F., Balsevich, J. and De Luca, V. Isolation and characterization of a 2-oxoglutarate dependent dioxygenase involved in the 2nd-to-last step in vindoline biosynthesis. Plant Physiol. 94 (1990) 1323–1329. [PMID: 16667836] |
| 2. |
De Carolis, E. and De Luca, V. Purification, characterization, and kinetic analysis of a 2-oxoglutarate-dependent dioxygenase involved in vindoline biosynthesis from Catharanthus roseus. J. Biol. Chem. 268 (1993) 5504–5511. [PMID: 8449913] |
| 3. |
Vazquez-Flota, F.A. and De Luca, V. Developmental and light regulation of desacetoxyvindoline 4-hydroxylase in Catharanthus roseus (L.) G. Don. Evidence of a multilevel regulatory mechanism. Plant Physiol. 117 (1998) 1351–1361. [PMID: 9701591] |
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| [EC 1.14.11.20 created 2002, modified 2005] |
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