ExplorEnz: EC 1.14.11.33

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1.14.11.33

Accepted name:

DNA oxidative demethylase

Reaction:

DNA-base-CH₃ + 2-oxoglutarate + O₂ = DNA-base + formaldehyde + succinate + CO₂

Other name(s):

alkylated DNA repair protein; α-ketoglutarate-dependent dioxygenase ABH1; alkB (gene name)

Systematic name:

methyl DNA-base, 2-oxoglutarate:oxygen oxidoreductase (formaldehyde-forming)

Comments:

Contains iron; activity is slightly stimulated by ascorbate. Catalyses oxidative demethylation of the DNA base lesions N¹-methyladenine, N³-methylcytosine, N¹-methylguanine, and N³-methylthymine. It works better on single-stranded DNA (ssDNA) and is capable of repairing damaged bases in RNA.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Falnes, P.O., Johansen, R.F. and Seeberg, E. AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli. Nature 419 (2002) 178–182. [DOI] [PMID: 12226668]
2.	Yi, C., Yang, C.G. and He, C. A non-heme iron-mediated chemical demethylation in DNA and RNA. Acc. Chem. Res. 42 (2009) 519–529. [DOI] [PMID: 19852088]
3.	Yi, C., Jia, G., Hou, G., Dai, Q., Zhang, W., Zheng, G., Jian, X., Yang, C.G., Cui, Q. and He, C. Iron-catalysed oxidation intermediates captured in a DNA repair dioxygenase. Nature 468 (2010) 330–333. [DOI] [PMID: 21068844]

[EC 1.14.11.33 created 2011]