| EC |
1.14.11.33 |
| Accepted name: |
DNA oxidative demethylase |
| Reaction: |
DNA-base-CH3 + 2-oxoglutarate + O2 = DNA-base + formaldehyde + succinate + CO2 |
| Other name(s): |
alkylated DNA repair protein; α-ketoglutarate-dependent dioxygenase ABH1; alkB (gene name) |
| Systematic name: |
methyl DNA-base, 2-oxoglutarate:oxygen oxidoreductase (formaldehyde-forming) |
| Comments: |
Contains iron; activity is slightly stimulated by ascorbate. Catalyses oxidative demethylation of the DNA base lesions N1-methyladenine, N3-methylcytosine, N1-methylguanine, and N3-methylthymine. It works better on single-stranded DNA (ssDNA) and is capable of repairing damaged bases in RNA. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
| References: |
| 1. |
Falnes, P.O., Johansen, R.F. and Seeberg, E. AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli. Nature 419 (2002) 178–182. [DOI] [PMID: 12226668] |
| 2. |
Yi, C., Yang, C.G. and He, C. A non-heme iron-mediated chemical demethylation in DNA and RNA. Acc. Chem. Res. 42 (2009) 519–529. [DOI] [PMID: 19852088] |
| 3. |
Yi, C., Jia, G., Hou, G., Dai, Q., Zhang, W., Zheng, G., Jian, X., Yang, C.G., Cui, Q. and He, C. Iron-catalysed oxidation intermediates captured in a DNA repair dioxygenase. Nature 468 (2010) 330–333. [DOI] [PMID: 21068844] |
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| [EC 1.14.11.33 created 2011] |
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