The Enzyme Database

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Accepted name: ectoine hydroxylase
Reaction: ectoine + 2-oxoglutarate + O2 = 5-hydroxyectoine + succinate + CO2
Glossary: ectoine = (4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylate
5-hydroxyectoine = (4S,5S)-5-hydroxy-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylate
Other name(s): ectD (gene name); ectoine dioxygenase
Systematic name: ectoine,2-oxoglutarate:oxygen oxidoreductase (5-hydroxylating)
Comments: Requires Fe2+ and ascorbate. The enzyme, found in bacteria, is specific for ectoine.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB
1.  Bursy, J., Pierik, A.J., Pica, N. and Bremer, E. Osmotically induced synthesis of the compatible solute hydroxyectoine is mediated by an evolutionarily conserved ectoine hydroxylase. J. Biol. Chem. 282 (2007) 31147–31155. [DOI] [PMID: 17636255]
2.  Bursy, J., Kuhlmann, A.U., Pittelkow, M., Hartmann, H., Jebbar, M., Pierik, A.J. and Bremer, E. Synthesis and uptake of the compatible solutes ectoine and 5-hydroxyectoine by Streptomyces coelicolor A3(2) in response to salt and heat stresses. Appl. Environ. Microbiol. 74 (2008) 7286–7296. [DOI] [PMID: 18849444]
3.  Reuter, K., Pittelkow, M., Bursy, J., Heine, A., Craan, T. and Bremer, E. Synthesis of 5-hydroxyectoine from ectoine: crystal structure of the non-heme iron(II) and 2-oxoglutarate-dependent dioxygenase EctD. PLoS One 5 (2010) e10647. [DOI] [PMID: 20498719]
[EC created 2017]

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