ExplorEnz: EC 1.14.12.22

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1.14.12.22

Accepted name:

carbazole 1,9a-dioxygenase

Reaction:

9H-carbazole + NAD(P)H + H⁺ + O₂ = 2′-aminobiphenyl-2,3-diol + NAD(P)⁺

Other name(s):

CARDO

Systematic name:

9H-carbazole,NAD(P)H:oxygen oxidoreductase (2,3-hydroxylating)

Comments:

This enzyme catalyses the first reaction in the pathway of carbazole degradation. The enzyme attacks at the 1 and 9a positions of carbazole, resulting in the formation of a highly unstable hemiaminal intermediate that undergoes a spontaneous cleavage and rearomatization, resulting in 2′-aminobiphenyl-2,3-diol. In most bacteria the enzyme is a complex composed of a terminal oxygenase, a ferredoxin, and a ferredoxin reductase. The terminal oxygenase component contains a nonheme iron centre and a Rieske [2Fe-2S] iron-sulfur cluster.

Links to other databases:

BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, PDB

References:

1.	Nam, J.W., Nojiri, H., Noguchi, H., Uchimura, H., Yoshida, T., Habe, H., Yamane, H. and Omori, T. Purification and characterization of carbazole 1,9a-dioxygenase, a three-component dioxygenase system of Pseudomonas resinovorans strain CA10. Appl. Environ. Microbiol. 68 (2002) 5882–5890. [DOI] [PMID: 12450807]
2.	Gai, Z., Wang, X., Liu, X., Tai, C., Tang, H., He, X., Wu, G., Deng, Z. and Xu, P. The genes coding for the conversion of carbazole to catechol are flanked by IS6100 elements in Sphingomonas sp. strain XLDN2-5. PLoS One 5:e10018 (2010). [DOI] [PMID: 20368802]

[EC 1.14.12.22 created 2010]