The Enzyme Database

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Accepted name: 4-methyl-5-nitrocatechol 5-monooxygenase
Reaction: 4-methyl-5-nitrocatechol + NAD(P)H + H+ + O2 = 2-hydroxy-5-methylquinone + nitrite + NAD(P)+ + H2O
Other name(s): dntB (gene name); 4-methyl-5-nitrocatechol oxygenase; MNC monooxygenase
Systematic name: 4-methyl-5-nitrocatechol,NAD(P)H:oxygen 5-oxidoreductase (5-hydroxylating, nitrite-forming)
Comments: Contains FAD. The enzyme, isolated from the bacterium Burkholderia sp. DNT, can use both NADH and NADPH, but prefers NADPH. It has a narrow substrate range, but can also act on 4-nitrocatechol.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
1.  Haigler, B.E., Suen, W.C. and Spain, J.C. Purification and sequence analysis of 4-methyl-5-nitrocatechol oxygenase from Burkholderia sp. strain DNT. J. Bacteriol. 178 (1996) 6019–6024. [DOI] [PMID: 8830701]
2.  Leungsakul, T., Johnson, G.R. and Wood, T.K. Protein engineering of the 4-methyl-5-nitrocatechol monooxygenase from Burkholderia sp. strain DNT for enhanced degradation of nitroaromatics. Appl. Environ. Microbiol. 72 (2006) 3933–3939. [DOI] [PMID: 16751499]
[EC created 2016]

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