ExplorEnz: EC 1.14.13.218

Your query returned 1 entry. Printable version

1.14.13.218

Accepted name:

5-methylphenazine-1-carboxylate 1-monooxygenase

Reaction:

5-methylphenazine-1-carboxylate + NADH + O₂ = pyocyanin + NAD⁺ + CO₂ + H₂O

For diagram of enediyne antitumour antibiotic biosynthesis and pyocyanin biosynthesis, click here

Glossary:

pyocyanin = 1-hydroxy-5-methylphenazin-5-ium

Other name(s):

phzS (gene name)

Systematic name:

5-methylphenazine-1-carboxylate,NADH:oxygen oxidoreductase (1-hydroxylating, decarboxylating)

Comments:

The enzyme, characterized from the bacterium Pseudomonas aeruginosa, is involved in the biosynthesis of pyocyanin, a toxin produced and secreted by the organism. It can also act on phenazine-1-carboxylate, converting it into phenazin-1-ol.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB

References:

1.	Mavrodi, D.V., Bonsall, R.F., Delaney, S.M., Soule, M.J., Phillips, G. and Thomashow, L.S. Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1. J. Bacteriol. 183 (2001) 6454–6465. [DOI] [PMID: 11591691]
2.	Parsons, J.F., Greenhagen, B.T., Shi, K., Calabrese, K., Robinson, H. and Ladner, J.E. Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa. Biochemistry 46 (2007) 1821–1828. [DOI] [PMID: 17253782]
3.	Greenhagen, B.T., Shi, K., Robinson, H., Gamage, S., Bera, A.K., Ladner, J.E. and Parsons, J.F. Crystal structure of the pyocyanin biosynthetic protein PhzS. Biochemistry 47 (2008) 5281–5289. [DOI] [PMID: 18416536]

[EC 1.14.13.218 created 2016]