The Enzyme Database

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EC 1.14.14.178     
Accepted name: steroid 22S-hydroxylase
Reaction: (1) a C27-steroid + O2 + [reduced NADPH—hemoprotein reductase] = a (22S)-22-hydroxy-C27-steroid + 2 H2O + [oxidized NADPH—hemoprotein reductase]
(2) a C28-steroid + O2 + [reduced NADPH—hemoprotein reductase] = a (22S)-22-hydroxy-C28-steroid + 2 H2O + [oxidized NADPH—hemoprotein reductase]
(3) a C29-steroid + O2 + [reduced NADPH—hemoprotein reductase] = a (22S)-22-hydroxy-C29-steroid + 2 H2O + [oxidized NADPH—hemoprotein reductase]
Other name(s): CYP90B1 (gene name); DWF4 (gene name); steroid C-22 hydroxylase
Systematic name: steroid,NADPH—hemoprotein reductase:oxygen 22S-oxidoreductase (hydroxylating)
Comments: This plant cytochrome P-450 (heme thiolate) enzyme participates in the biosynthesis of brassinosteroids. While in vivo substrates include C28-steroids such as campestanol, campesterol, and 6-oxocampestanol, the enzyme is able to catalyse the C-22 hydroxylation of a variety of C27, C28 and C29 steroids.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Asami, T., Mizutani, M., Fujioka, S., Goda, H., Min, Y.K., Shimada, Y., Nakano, T., Takatsuto, S., Matsuyama, T., Nagata, N., Sakata, K. and Yoshida, S. Selective interaction of triazole derivatives with DWF4, a cytochrome P450 monooxygenase of the brassinosteroid biosynthetic pathway, correlates with brassinosteroid deficiency in planta. J. Biol. Chem. 276 (2001) 25687–25691. [DOI] [PMID: 11319239]
2.  Choe, S., Fujioka, S., Noguchi, T., Takatsuto, S., Yoshida, S. and Feldmann, K.A. Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis. Plant J. 26 (2001) 573–582. [DOI] [PMID: 11489171]
3.  Asami, T., Mizutani, M., Shimada, Y., Goda, H., Kitahata, N., Sekimata, K., Han, S.Y., Fujioka, S., Takatsuto, S., Sakata, K. and Yoshida, S. Triadimefon, a fungicidal triazole-type P450 inhibitor, induces brassinosteroid deficiency-like phenotypes in plants and binds to DWF4 protein in the brassinosteroid biosynthesis pathway. Biochem. J. 369 (2003) 71–76. [DOI] [PMID: 12350224]
4.  Fujita, S., Ohnishi, T., Watanabe, B., Yokota, T., Takatsuto, S., Fujioka, S., Yoshida, S., Sakata, K. and Mizutani, M. Arabidopsis CYP90B1 catalyses the early C-22 hydroxylation of C27, C28 and C29 sterols. Plant J. 45 (2006) 765–774. [DOI] [PMID: 16460510]
5.  Ohnishi, T., Watanabe, B., Sakata, K. and Mizutani, M. CYP724B2 and CYP90B3 function in the early C-22 hydroxylation steps of brassinosteroid biosynthetic pathway in tomato. Biosci. Biotechnol. Biochem. 70 (2006) 2071–2080. [DOI] [PMID: 16960392]
[EC 1.14.14.178 created 2022]
 
 


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