ExplorEnz: EC 1.14.14.27

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1.14.14.27

Accepted name:

resorcinol 4-hydroxylase (FADH₂)

Reaction:

resorcinol + FADH₂ + O₂ = hydroxyquinol + FAD + H₂O

Glossary:

resorcinol = benzene-1,3-diol
hydroxyquinol = benzene-1,2,4-triol

Other name(s):

graA (gene name)

Systematic name:

resorcinol,FADH₂:oxygen oxidoreductase (4-hydroxylating)

Comments:

The enzyme, characterized from the bacterium Rhizobium sp. strain MTP-10005, uses FADH₂ as a substrate rather than a cofactor. FADH₂ is provided by a dedicated EC 1.5.1.36, flavin reductase (NADH). The enzyme participates in the degradation of γ-resorcylate and resorcinol. cf. EC 1.14.13.220, resorcinol 4-hydroxylase (NADH), and EC 1.14.13.219, resorcinol 4-hydroxylase (NADPH).

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc

References:

1.	Ohta, Y. and Ribbons, D.W. Bacterial metabolism of resorcinylic compounds: purification and properties of orcinol hydroxylase and resorcinol hydroxylase from Pseudomonas putida ORC. Eur. J. Biochem. 61 (1976) 259–269. [DOI] [PMID: 1280]
2.	Yoshida, M., Oikawa, T., Obata, H., Abe, K., Mihara, H. and Esaki, N. Biochemical and genetic analysis of the γ-resorcylate (2,6-dihydroxybenzoate) catabolic pathway in Rhizobium sp. strain MTP-10005: identification and functional analysis of its gene cluster. J. Bacteriol. 189 (2007) 1573–1581. [DOI] [PMID: 17158677]

[EC 1.14.14.27 created 2016]