| EC |
1.14.14.88 |
| Accepted name: |
isoflavone 3′-hydroxylase |
| Reaction: |
formononetin + [reduced NADPH—hemoprotein reductase] + O2 = calycosin + [oxidized NADPH—hemoprotein reductase] + H2O |
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For diagram of medicarpin and formononetin derivatives biosynthesis, click here |
| Glossary: |
calycosin = 3′-hydroxyformononetin |
| Other name(s): |
isoflavone 3′-monooxygenase; CYP81E9 |
| Systematic name: |
formononetin,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (3′-hydroxylating) |
| Comments: |
A cytochrome P-450 (heme-thiolate) protein. Also acts on biochanin A and other isoflavones with a 4′-methoxy group. Involved in the biosynthesis of the pterocarpin phytoalexins medicarpin and maackiain. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 110183-50-1 |
| References: |
| 1. |
Hinderer, W., Flentje, U. and Barz, W. Microsomal isoflavone 2′-hydroxylases and 3′-hydroxylases from chickpea (Cicer arietinum L) cell-suspensions induced for pterocarpan phytoalexin formation. FEBS Lett. 214 (1987) 101–106. |
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| [EC 1.14.14.88 created 1992 as EC 1.14.13.52, transferred 2018 to EC 1.14.14.88] |
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