ExplorEnz: EC 1.14.15.22

Your query returned 1 entry. Printable version

1.14.15.22

Accepted name:

vitamin D 1,25-hydroxylase

Reaction:

(1) calciol + O₂ + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H⁺ = calcidiol + 2 oxidized ferredoxin [iron-sulfur] cluster + H₂O
(2) calcidiol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H⁺ + O₂ = calcitriol + 2 oxidized ferredoxin [iron-sulfur] cluster + H₂O

Glossary:

calciol = cholecalciferol = vitamin D₃ = (3S,5Z,7E)-9,10-seco-5,7,10(19)-cholestatriene-3-ol
calcidiol = 25-hydroxyvitamin D₃ = (3S,5Z,7E)-9,10-seco-5,7,10(19)-cholestatriene-3,25-diol
calcitriol = 1α,25-dihydroxyvitamin D₃ = (1S,3R,5Z,7E)-9,10-seco-5,7,10(19)-cholestatriene-1,3,25-triol

Other name(s):

CYP105A1; Streptomyces griseolus cytochrome P450SU-1

Systematic name:

calciol,ferredoxin:oxygen oxidoreductase (1,25-hydroxylating)

Comments:

A P-450 (heme-thiolate) enzyme found in the bacterium Streptomyces griseolus. cf. EC 1.14.14.24, vitamin D 25-hydroxylase and EC 1.14.15.18, calcidiol 1-monooxygenase.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB

References:

1.	Sawada, N., Sakaki, T., Yoneda, S., Kusudo, T., Shinkyo, R., Ohta, M. and Inouye, K. Conversion of vitamin D₃ to 1α,25-dihydroxyvitamin D₃ by Streptomyces griseolus cytochrome P450SU-1. Biochem. Biophys. Res. Commun. 320 (2004) 156–164. [DOI] [PMID: 15207715]
2.	Sugimoto, H., Shinkyo, R., Hayashi, K., Yoneda, S., Yamada, M., Kamakura, M., Ikushiro, S., Shiro, Y. and Sakaki, T. Crystal structure of CYP105A1 (P450SU-1) in complex with 1α,25-dihydroxyvitamin D₃. Biochemistry 47 (2008) 4017–4027. [DOI] [PMID: 18314962]

[EC 1.14.15.22 created 2016]