ExplorEnz: EC 1.14.18.2

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1.14.18.2

Accepted name:

CMP-N-acetylneuraminate monooxygenase

Reaction:

CMP-N-acetylneuraminate + 2 ferrocytochrome b₅ + O₂ + 2 H⁺ = CMP-N-glycoloylneuraminate + 2 ferricytochrome b₅ + H₂O

Other name(s):

CMP-N-acetylneuraminic acid hydroxylase; CMP-Neu5Ac hydroxylase; cytidine monophosphoacetylneuraminate monooxygenase; N-acetylneuraminic monooxygenase; cytidine-5′-monophosphate-N-acetylneuraminic acid hydroxylase

Systematic name:

CMP-N-acetylneuraminate,ferrocytochrome-b₅:oxygen oxidoreductase (N-acetyl-hydroxylating)

Comments:

This enzyme contains both a Rieske-type [2Fe-2S] cluster and a second iron site. The ferricytochrome b₅ produced is reduced by NADH and cytochrome-b₅ reductase (EC 1.6.2.2). The enzyme can be activated by Fe²⁺ or Fe³⁺.

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, CAS registry number: 116036-67-0

References:

1.	Shaw, L. and Schauer, R. The biosynthesis of N-glycoloylneuraminic acid occurs by hydroxylation of the CMP-glycoside of N-acetylneuraminic acid. Biol. Chem. Hoppe-Seyler 369 (1988) 477–486. [PMID: 3202954]
2.	Kozutsumi, Y., Kawano, T., Yamakawa, T. and Suzuki, A. Participation of cytochrome b₅ in CMP-N-acetylneuraminic acid hydroxylation in mouse liver cytosol. J. Biochem. (Tokyo) 109 (1990) 704–706. [PMID: 1964451]
3.	Schneckenburger, P., Shaw, L. and Schauer, R. Purification, characterization and reconstitution of CMP-N-acetylneuraminate hydroxylase from mouse liver. Glycoconj. J. 11 (1994) 194–203. [PMID: 7841794]
4.	Kawano, T., Koyama, S., Takematsu, H., Kozutsumi, Y., Kawasaki, H., Kawashima, S., Kawasaki, T. and Suzuki, A. Molecular cloning of cytidine monophospho-N-acetylneuraminic acid hydroxylase. Regulation of species- and tissue-specific expression of N-glycolylneuraminic acid. J. Biol. Chem. 270 (1995) 16458–16463. [DOI] [PMID: 7608218]
5.	Schlenzka, W., Shaw, L., Kelm, S., Schmidt, C.L., Bill, E., Trautwein, A.X., Lottspeich, F. and Schauer, R. CMP-N-acetylneuraminic acid hydroxylase: the first cytosolic Rieske iron-sulphur protein to be described in Eukarya. FEBS Lett. 385 (1996) 197–200. [DOI] [PMID: 8647250]

[EC 1.14.18.2 created 1992 as EC 1.14.13.45, transferred 2003 to EC 1.14.18.2]