ExplorEnz: EC 1.14.20.1

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1.14.20.1

Accepted name:

deacetoxycephalosporin-C synthase

Reaction:

penicillin N + 2-oxoglutarate + O₂ = deacetoxycephalosporin C + succinate + CO₂ + H₂O

For diagram of penicillin-N and deacetoxycephalosporin-C biosynthesis, click here

Other name(s):

DAOCS; penicillin N expandase; DAOC synthase

Systematic name:

penicillin-N,2-oxoglutarate:oxygen oxidoreductase (ring-expanding)

Comments:

Forms part of the penicillin biosynthesis pathway (for pathway, click here).

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 85746-10-7

References:

1.	Cantwell, C., Beckmann, R., Whiteman, P., Queener, S.W. and Abraham, E.P. Isolation of deacetoxycephalosporin-c from fermentation broths of Penicillium chrysogenum transformants - construction of a new fungal biosynthetic-pathway. Proc. R. Soc. Lond. B Biol. Sci. 248 (1992) 283–289. [DOI] [PMID: 1354366]
2.	Lee, H.J., Lloyd, M.D., Harlos, K., Clifton, I.J., Baldwin, J.E. and Schofield, C.J. Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS). J. Mol. Biol. 308 (2001) 937–948. [DOI] [PMID: 11352583]
3.	Yeh, W.K., Ghag, S.K. and Queener, S.W. Enzymes for epimerization of isopenicillin N, ring expansion of penicillin N, and 3′-hydroxylation of deacetoxycephalosporin C. Function, evolution, refolding, and enzyme engineering. Ann. N.Y. Acad. Sci. 672 (1992) 396–408.
4.	Valegaard, K., van Scheltinga, A.C.T., Lloyd, M.D., Hara, T., Ramaswamy, S., Perrakis, A., Thompson, A., Lee, H.-J., Baldwin, J.E., Schofield, C.J., Hajdu, J. and Andersson, I. Structure of a cephalosporin synthase. Nature 394 (1998) 805–809. [DOI] [PMID: 9723623]
5.	Dotzlaf, J.E. and Yeh, W.K. Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus. J. Biol. Chem. 264 (1989) 10219–10227. [PMID: 2656705]

[EC 1.14.20.1 created 2002]