| EC |
1.14.99.15 |
| Accepted name: |
4-methoxybenzoate monooxygenase (O-demethylating) |
| Reaction: |
4-methoxybenzoate + reduced acceptor + O2 = 4-hydroxybenzoate + formaldehyde + acceptor + H2O |
| Other name(s): |
4-methoxybenzoate 4-monooxygenase (O-demethylating); 4-methoxybenzoate O-demethylase; p-anisic O-demethylase; piperonylate-4-O-demethylase |
| Systematic name: |
4-methoxybenzoate,hydrogen-donor:oxygen oxidoreductase (O-demethylating) |
| Comments: |
The bacterial enzyme consists of a ferredoxin-type protein and an iron-sulfur flavoprotein (FMN). Also acts on 4-ethoxybenzoate, N-methyl-4-aminobenzoate and toluate. The fungal enzyme acts best on veratrate. |
| Links to other databases: |
BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 37256-78-3 |
| References: |
| 1. |
Bernhardt, F.-H., Nastainczyk, W. and Seydewitz, V. Kinetic studies on a 4-methoxybenzoate O-demethylase from Pseudomonas putida. Eur. J. Biochem. 72 (1977) 107–115. [DOI] [PMID: 188654] |
| 2. |
Paszcynski, A. and Trojanowski, J. An affinity-column procedure for the purification of veratrate O-demethylase from fungi. Microbios 18 (1977) 111–121. [PMID: 25369] |
| 3. |
Twilfer, H., Bernhardt, F.-H. and Gersonde, K. An electron-spin-resonance study on the redox-active centers of the 4-methoxybenzoate monooxygenase from Pseudomonas putida. Eur. J. Biochem. 119 (1981) 595–602. [DOI] [PMID: 6273164] |
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| [EC 1.14.99.15 created 1972] |
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