ExplorEnz: EC 1.14.99.22

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1.14.99.22

Accepted name:

ecdysone 20-monooxygenase

Reaction:

ecdysone + reduced acceptor + O₂ = 20-hydroxyecdysone + acceptor + H₂O

Other name(s):

α-ecdysone C-20 hydroxylase; ecdysone 20-hydroxylase

Systematic name:

Ecdysone,hydrogen-donor:oxygen oxidoreductase (20-hydroxylating)

Comments:

An enzyme from insect fat body or malpighian tubules involving a heme-thiolate protein (P-450). NADPH can act as ultimate hydrogen donor.

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, CAS registry number: 55071-97-1

References:

1.	Johnson, P. and Rees, H.H. The mechanism of C-20 hydroxylation of α-ecdysone in the desert locust, Schistocerca gregaria. Biochem. J. 168 (1977) 513–520. [PMID: 606249]
2.	Nigg, H.N., Svoboda, J.A., Thompson, M.J., Dutky, S.R., Kaplanis, J.N. and Robbins, W.E. Ecdysome 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.). Experientia 32 (1976) 438–439. [PMID: 5286]
3.	Smith, S.L., Bollenbacher, W.E., Cooper, D.Y., Schleyer, H., Wielgus, J.J. and Gilbert, L.I. Ecdysone 20-monooxygenase: characterization of an insect cytochrome p-450 dependent steroid hydroxylase. Mol. Cell. Endocrinol. 15 (1979) 111–133. [PMID: 488526]

[EC 1.14.99.22 created 1978]