The Enzyme Database

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Accepted name: tRNA 2-(methylsulfanyl)-N6-isopentenyladenosine37 hydroxylase
Reaction: 2-(methylsulfanyl)-N6-prenyladenosine37 in tRNA + reduced acceptor + O2 = N6-[(2E)-4-hydroxy-3-methylbut-2-en-1-yl]-2-(methylsulfanyl)adenosine37 in tRNA + acceptor + H2O
Glossary: 2-(methylsulfanyl)-N6-prenyladenosine = N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenosine
Other name(s): miaE (gene name); tRNA 2-methylthio-N6-isopentenyl adenosine(37) hydroxylase; tRNA 2-(methylsulfanyl)-N6-dimethylallyladenosine37 hydroxylase
Systematic name: tRNA 2-(methylsulfanyl)-N6-prenyladenosine37,donor:oxygen 4-oxidoreductase (trans-hydroxylating)
Comments: The enzyme, found only within a small subset of facultative anaerobic bacteria, belongs to the nonheme diiron family. The enzyme from Salmonella typhimurium was shown to catalyse a stereoselective (E)-hydroxylation at the terminal C4-position of the prenyl group.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB
1.  Persson, B.C. and Bjork, G.R. Isolation of the gene (miaE) encoding the hydroxylase involved in the synthesis of 2-methylthio-cis-ribozeatin in tRNA of Salmonella typhimurium and characterization of mutants. J. Bacteriol. 175 (1993) 7776–7785. [DOI] [PMID: 8253666]
2.  Persson, B.C., Olafsson, O., Lundgren, H.K., Hederstedt, L. and Bjork, G.R. The ms2io6A37 modification of tRNA in Salmonella typhimurium regulates growth on citric acid cycle intermediates. J. Bacteriol. 180 (1998) 3144–3151. [DOI] [PMID: 9620964]
3.  Corder, A.L., Subedi, B.P., Zhang, S., Dark, A.M., Foss, F.W., Jr. and Pierce, B.S. Peroxide-shunt substrate-specificity for the Salmonella typhimurium O2-dependent tRNA modifying monooxygenase (MiaE). Biochemistry 52 (2013) 6182–6196. [DOI] [PMID: 23906247]
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