| EC |
1.14.99.69 |
| Accepted name: |
tRNA 2-(methylsulfanyl)-N6-isopentenyladenosine37 hydroxylase |
| Reaction: |
2-(methylsulfanyl)-N6-prenyladenosine37 in tRNA + reduced acceptor + O2 = N6-[(2E)-4-hydroxy-3-methylbut-2-en-1-yl]-2-(methylsulfanyl)adenosine37 in tRNA + acceptor + H2O |
| Glossary: |
2-(methylsulfanyl)-N6-prenyladenosine = N6-(3-methylbut-2-en-1-yl)-2-(methylsulfanyl)adenosine |
| Other name(s): |
miaE (gene name); tRNA 2-methylthio-N6-isopentenyl adenosine(37) hydroxylase; tRNA 2-(methylsulfanyl)-N6-dimethylallyladenosine37 hydroxylase |
| Systematic name: |
tRNA 2-(methylsulfanyl)-N6-prenyladenosine37,donor:oxygen 4-oxidoreductase (trans-hydroxylating) |
| Comments: |
The enzyme, found only within a small subset of facultative anaerobic bacteria, belongs to the nonheme diiron family. The enzyme from Salmonella typhimurium was shown to catalyse a stereoselective (E)-hydroxylation at the terminal C4-position of the prenyl group. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
| References: |
| 1. |
Persson, B.C. and Bjork, G.R. Isolation of the gene (miaE) encoding the hydroxylase involved in the synthesis of 2-methylthio-cis-ribozeatin in tRNA of Salmonella typhimurium and characterization of mutants. J. Bacteriol. 175 (1993) 7776–7785. [DOI] [PMID: 8253666] |
| 2. |
Persson, B.C., Olafsson, O., Lundgren, H.K., Hederstedt, L. and Bjork, G.R. The ms2io6A37 modification of tRNA in Salmonella typhimurium regulates growth on citric acid cycle intermediates. J. Bacteriol. 180 (1998) 3144–3151. [DOI] [PMID: 9620964] |
| 3. |
Corder, A.L., Subedi, B.P., Zhang, S., Dark, A.M., Foss, F.W., Jr. and Pierce, B.S. Peroxide-shunt substrate-specificity for the Salmonella typhimurium O2-dependent tRNA modifying monooxygenase (MiaE). Biochemistry 52 (2013) 6182–6196. [DOI] [PMID: 23906247] |
|
| [EC 1.14.99.69 created 2020] |
| |
|
| |
|
Printable version