ExplorEnz: EC 1.17.98.2

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1.17.98.2

Accepted name:

bacteriochlorophyllide c C-7¹-hydroxylase

Reaction:

2 S-adenosyl-L-methionine + a bacteriochlorophyllide c + H₂O = a bacteriochlorophyllide e + 2 5′-deoxyadenosine + 2 L-methionine (overall reaction)
(1a) S-adenosyl-L-methionine + a bacteriochlorophyllide c + H₂O = a 7-(hydroxymethyl)bacteriochlorophyllide c + 5′-deoxyadenosine + L-methionine
(1b) S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H₂O = a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5′-deoxyadenosine + L-methionine
(1c) a 7-(dihydroxymethyl)bacteriochlorophyllide c = a bacteriochlorophyllide e + H₂O (spontaneous)

For diagram of botryococcus braunii BOT22 squalene and botrycoccene biosynthesis, click here

Other name(s):

bciD (gene name)

Systematic name:

bacteriochlorophyllide-c:S-adenosyl-L-methionine oxidoreductase (C-7¹-hydroxylating)

Comments:

The enzyme, found in green sulfur bacteria (Chlorobiaceae), is a radical S-adenosyl-L-methionine (AdoMet) enzyme and contains a [4Fe-4S] cluster. It catalyses two consecutive hydroxylation reactions of the C-7 methyl group of bacteriochlorophyllide c to form a geminal diol intermediate that spontaneously dehydrates to produce the formyl group of bacteriochlorophyllide e.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Harada, J., Mizoguchi, T., Satoh, S., Tsukatani, Y., Yokono, M., Noguchi, M., Tanaka, A. and Tamiaki, H. Specific gene bciD for C7-methyl oxidation in bacteriochlorophyll e biosynthesis of brown-colored green sulfur bacteria. PLoS One 8:e60026 (2013). [DOI] [PMID: 23560066]
2.	Thweatt, J.L., Ferlez, B.H., Golbeck, J.H. and Bryant, D.A. BciD is a radical S-adenosyl-L-methionine (SAM) enzyme that completes bacteriochlorophyllide e biosynthesis by oxidizing a methyl group into a formyl group at C-7. J. Biol. Chem. 292 (2017) 1361–1373. [DOI] [PMID: 27994052]

[EC 1.17.98.2 created 2016, modified 2017]