| EC |
1.3.1.85 |
| Accepted name: |
crotonyl-CoA carboxylase/reductase |
| Reaction: |
(2S)-ethylmalonyl-CoA + NADP+ = (E)-but-2-enoyl-CoA + CO2 + NADPH + H+ |
| Glossary: |
(E)-but-2-enoyl-CoA = crotonyl-CoA |
| Other name(s): |
CCR; crotonyl-CoA reductase (carboxylating) |
| Systematic name: |
(2S)-ethylmalonyl-CoA:NADP+ oxidoreductase (decarboxylating) |
| Comments: |
The reaction is catalysed in the reverse direction. This enzyme, isolated from the bacterium Rhodobacter sphaeroides, catalyses (E)-but-2-enoyl-CoA-dependent oxidation of NADPH in the presence of CO2. When CO2 is absent, the enzyme catalyses the reduction of (E)-but-2-enoyl-CoA to butanoyl-CoA, but with only 10% of maximal activity (relative to (E)-but-2-enoyl-CoA carboxylation). |
| Links to other databases: |
BRENDA, EXPASY, Gene, KEGG, MetaCyc |
| References: |
| 1. |
Erb, T.J., Berg, I.A., Brecht, V., Muller, M., Fuchs, G. and Alber, B.E. Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway. Proc. Natl. Acad. Sci. USA 104 (2007) 10631–10636. [DOI] [PMID: 17548827] |
| 2. |
Erb, T.J., Brecht, V., Fuchs, G., Muller, M. and Alber, B.E. Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase. Proc. Natl. Acad. Sci. USA 106 (2009) 8871–8876. [DOI] [PMID: 19458256] |
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| [EC 1.3.1.85 created 2011] |
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