ExplorEnz: EC 1.4.3.13

Your query returned 1 entry. Printable version

1.4.3.13

Accepted name:

protein-lysine 6-oxidase

Reaction:

[protein]-L-lysine + O₂ + H₂O = [protein]-(S)-2-amino-6-oxohexanoate + NH₃ + H₂O₂

Glossary:

(S)-2-amino-6-oxohexanoate = L-allysine

Other name(s):

lysyl oxidase

Systematic name:

protein-L-lysine:oxygen 6-oxidoreductase (deaminating)

Comments:

Also acts on protein 5-hydroxylysine. This enzyme catalyses the final known enzymic step required for collagen and elastin cross-linking in the biosynthesis of normal mature extracellular matrices [4]. These reactions play an important role for the development, elasticity and extensibility of connective tissue. The enzyme is also active on free amines, such as cadaverine or benzylamine [4,5]. Some isoforms can also use [protein]-N(6)-acetyl-L-lysine as substrate deacetamidating the substrate [6].

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 99676-44-5

References:

1.	Harris, E.D., Gonnerman, W.A., Savage, J.E. and O'Dell, B.L. Connective tissue amine oxidase. II. Purification and partial characterization of lysyl oxidase from chick aorta. Biochim. Biophys. Acta 341 (1974) 332–344. [DOI] [PMID: 4838158]
2.	Rayton, J.K. and Harris, E.D. Induction of lysyl oxidase with copper. Properties of an in vitro system. J. Biol. Chem. 254 (1979) 621–626. [PMID: 33171]
3.	Stassen, F.L.H. Properties of highly purified lysyl oxidase from embryonic chick cartilage. Biochim. Biophys. Acta 438 (1976) 49–60. [DOI] [PMID: 7318]
4.	Palamakumbura, A.H. and Trackman, P.C. A fluorometric assay for detection of lysyl oxidase enzyme activity in biological samples. Anal. Biochem. 300 (2002) 245–251. [DOI] [PMID: 11779117]
5.	Kagan, H.M., Williams, M.A., Williamson, P.R. and Anderson, J.M. Influence of sequence and charge on the specificity of lysyl oxidase toward protein and synthetic peptide substrates. J. Biol. Chem. 259 (1984) 11203–11207. [PMID: 6147351]
6.	Rodriguez, H.M., Vaysberg, M., Mikels, A., McCauley, S., Velayo, A.C., Garcia, C. and Smith, V. Modulation of lysyl oxidase-like 2 enzymatic activity by an allosteric antibody inhibitor. J. Biol. Chem. 285 (2010) 20964–20974. [DOI] [PMID: 20439985]
7.	Kim, Y.M., Kim, E.C. and Kim, Y. The human lysyl oxidase-like 2 protein functions as an amine oxidase toward collagen and elastin. Mol. Biol. Rep. 38 (2011) 145–149. [DOI] [PMID: 20306300]
8.	Xu, L., Go, E.P., Finney, J., Moon, H., Lantz, M., Rebecchi, K., Desaire, H. and Mure, M. Post-translational modifications of recombinant human lysyl oxidase-like 2 (rhLOXL2) secreted from Drosophila S2 cells. J. Biol. Chem. 288 (2013) 5357–5363. [DOI] [PMID: 23319596]
9.	Ma, L., Huang, C., Wang, X.J., Xin, D.E., Wang, L.S., Zou, Q.C., Zhang, Y.S., Tan, M.D., Wang, Y.M., Zhao, T.C., Chatterjee, D., Altura, R.A., Wang, C., Xu, Y.S., Yang, J.H., Fan, Y.S., Han, B.H., Si, J., Zhang, X., Cheng, J., Chang, Z. and Chin, Y.E. Lysyl oxidase 3 is a dual-specificity enzyme involved in STAT3 deacetylation and deacetylimination modulation. Mol. Cell 65 (2017) 296–309. [PMID: 28065600]

[EC 1.4.3.13 created 1980, modified 1983]