ExplorEnz: EC 1.5.3.13

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1.5.3.13

Accepted name:

N¹-acetylpolyamine oxidase

Reaction:

(1) N¹-acetylspermidine + O₂ + H₂O = putrescine + 3-acetamidopropanal + H₂O₂
(2) N¹-acetylspermine + O₂ + H₂O = spermidine + 3-acetamidopropanal + H₂O₂

Other name(s):

hPAO-1; PAO (ambiguous); mPAO; hPAO; polyamine oxidase (ambiguous)

Systematic name:

N¹-acetylpolyamine:oxygen oxidoreductase (3-acetamidopropanal-forming)

Comments:

The enzyme also catalyses the reaction: N¹,N¹²-diacetylspermine + O₂ + H₂O = N¹-acetylspermidine + 3-acetamamidopropanal + H₂O₂ [1]. No or very weak activity with spermine, or spermidine in absence of aldehydes. In presence of aldehydes the enzyme catalyses the reactions: 1. spermine + O₂ + H₂O = spermidine + 3-aminopropanal + H₂O₂, and with weak efficiency 2. spermidine + O₂ + H₂O = putrescine + 3-aminopropanal + H₂O₂ [2]. A flavoprotein (FAD). This enzyme, encoded by the PAOX gene, is found in mammalian peroxisomes and oxidizes N¹-acetylated polyamines at the exo (three-carbon) side of the secondary amine, forming 3-acetamamidopropanal. Since the products of the reactions are deacetylated polyamines, this process is known as polyamine back-conversion. Differs in specificity from EC 1.5.3.14 [polyamine oxidase (propane-1,3-diamine-forming)], EC 1.5.3.15 [N⁸-acetylspermidine oxidase (propane-1,3-diamine-forming)], EC 1.5.3.16 (spermine oxidase) and EC 1.5.3.17 (non-specific polyamine oxidase).

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB

References:

1.	Vujcic, S., Liang, P., Diegelman, P., Kramer, D.L. and Porter, C.W. Genomic identification and biochemical characterization of the mammalian polyamine oxidase involved in polyamine back-conversion. Biochem. J. 370 (2003) 19–28. [DOI] [PMID: 12477380]
2.	Jarvinen, A., Grigorenko, N., Khomutov, A.R., Hyvonen, M.T., Uimari, A., Vepsalainen, J., Sinervirta, R., Keinanen, T.A., Vujcic, S., Alhonen, L., Porter, C.W. and Janne, J. Metabolic stability of α-methylated polyamine derivatives and their use as substitutes for the natural polyamines. J. Biol. Chem. 280 (2005) 6595–6601. [DOI] [PMID: 15611107]
3.	Wang, Y., Hacker, A., Murray-Stewart, T., Frydman, B., Valasinas, A., Fraser, A.V., Woster, P.M. and Casero, R.A., Jr. Properties of recombinant human N¹-acetylpolyamine oxidase (hPAO): potential role in determining drug sensitivity. Cancer Chemother. Pharmacol. 56 (2005) 83–90. [DOI] [PMID: 15791459]
4.	Wu, T., Yankovskaya, V. and McIntire, W.S. Cloning, sequencing, and heterologous expression of the murine peroxisomal flavoprotein, N¹-acetylated polyamine oxidase. J. Biol. Chem. 278 (2003) 20514–20525. [DOI] [PMID: 12660232]

[EC 1.5.3.13 created 2009]