| EC |
1.8.1.5 |
| Accepted name: |
2-oxopropyl-CoM reductase (carboxylating) |
| Reaction: |
CoM + acetoacetate + NADP+ = 2-oxopropyl-CoM + CO2 + NADPH |
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For diagram of epoxide carboxylation, click here |
| Glossary: |
coenzyme M (CoM) = 2-sulfanylethane-1-sulfonate = 2-mercaptoethanesulfonate (deprecated) |
| Other name(s): |
NADPH:2-(2-ketopropylthio)ethanesulfonate oxidoreductase/carboxylase; NADPH:2-ketopropyl-coenzyme M oxidoreductase/carboxylase; 2-mercaptoethanesulfonate,acetoacetate:NADP+ oxidoreductase (decarboxylating) |
| Systematic name: |
2-sulfanylethane-1-sulfonate,acetoacetate:NADP+ oxidoreductase (decarboxylating) |
| Comments: |
Also acts on thioethers longer in chain length on the oxo side, e.g. 2-oxobutyl-CoM, but this portion must be attached to CoM (2-sulfanylethane-1-sulfonate); no CoM analogs will substitute. This enzyme forms component II of a four-component enzyme system EC 4.4.1.23 (2-hydroxypropyl-CoM lyase; component I), EC 1.8.1.5 [2-oxopropyl-CoM reductase (carboxylating); component II], EC 1.1.1.268 [2-(R)-hydroxypropyl-CoM dehydrogenase; component III] and EC 1.1.1.269 [2-(S)-hydroxypropyl-CoM dehydrogenase; component IV].html">click here that is involved in epoxyalkane carboxylation in Xanthobacter sp. strain Py2. |
| Links to other databases: |
BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 244301-63-1 |
| References: |
| 1. |
Allen, J.R., Clark, D.D., Krum, J.G. and Ensign, S.A. A role for coenzyme M (2-mercaptoethanesulfonic acid) in a bacterial pathway of aliphatic epoxide carboxylation. Proc. Natl. Acad. Sci. USA 96 (1999) 8432–8437. [DOI] [PMID: 10411892] |
| 2. |
Clark, D.D., Allen, J.R. and Ensign, S.A. Characterization of five catalytic activities associated with the NADPH:2-ketopropyl-coenzyme M [2-(2-ketopropylthio)ethanesulfonate] oxidoreductase/carboxylase of the Xanthobacter strain Py2 epoxide carboxylase system. Biochemistry 39 (2000) 1294–1304. [DOI] [PMID: 10684609] |
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| [EC 1.8.1.5 created 2001] |
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