ExplorEnz: EC 1.8.98.7

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1.8.98.7

Accepted name:

cysteine-type anaerobic sulfatase-maturating enzyme

Reaction:

S-adenosyl-L-methionine + a [sulfatase]-L-cysteine + H₂O = a [sulfatase]-3-oxo-L-alanine + 5′-deoxyadenosine + L-methionine + hydrogen sulfide

Glossary:

3-oxo-L-alanine = formylglycine = Cα-formylglycine = FGly

Other name(s):

anSME; Cys-type anaerobic sulfatase-maturating enzyme; anaerobic sulfatase maturase

Systematic name:

[sulfatase]-L-cysteine:S-adenosyl-L-methionine oxidoreductase (3-oxo-L-alanine-forming)

Comments:

A radical S-adenosylmethionine (AdoMet) enzyme that contains three [4Fe-4S] clusters. The enzyme, found in some bacteria, activates a type I sulfatase enzyme (EC 3.1.6.1) by converting a conserved L-cysteine residue in the active site to a unique 3-oxo-L-alanine residue that is essential for the sulfatase activity. Some enzymes can also act on L-serine, see EC 1.1.98.7, serine-type anaerobic sulfatase-maturating enzyme and EC 1.8.3.7, formylglycine-generating enzyme.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB

References:

1.	Berteau, O., Guillot, A., Benjdia, A. and Rabot, S. A new type of bacterial sulfatase reveals a novel maturation pathway in prokaryotes. J. Biol. Chem. 281 (2006) 22464–22470. [PMID: 16766528]
2.	Benjdia, A., Subramanian, S., Leprince, J., Vaudry, H., Johnson, M.K. and Berteau, O. Anaerobic sulfatase-maturating enzymes, first dual substrate radical S-adenosylmethionine enzymes. J. Biol. Chem. 283 (2008) 17815–17826. [PMID: 18408004]
3.	Benjdia, A., Leprince, J., Sandstrom, C., Vaudry, H. and Berteau, O. Mechanistic investigations of anaerobic sulfatase-maturating enzyme: direct Cβ H-atom abstraction catalyzed by a radical AdoMet enzyme. J. Am. Chem. Soc. 131 (2009) 8348–8349. [PMID: 19489556]
4.	Benjdia, A., Subramanian, S., Leprince, J., Vaudry, H., Johnson, M.K. and Berteau, O. Anaerobic sulfatase-maturating enzyme^--a mechanistic link with glycyl radical-activating enzymes. FEBS J. 277 (2010) 1906–1920. [PMID: 20218986]
5.	Grove, T.L., Ahlum, J.H., Qin, R.M., Lanz, N.D., Radle, M.I., Krebs, C. and Booker, S.J. Further characterization of Cys-type and Ser-type anaerobic sulfatase maturating enzymes suggests a commonality in the mechanism of catalysis. Biochemistry 52 (2013) 2874–2887. [PMID: 23477283]

[EC 1.8.98.7 created 2020]