ExplorEnz: EC 1.97.1.9

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1.97.1.9

Accepted name:

selenate reductase (cytochrome c)

Reaction:

2 ferricytochrome c + selenite + H₂O = 2 ferrocytochrome c + selenate

Other name(s):

serA (gene name); serB (gene name); serC (gene name); selenite:reduced acceptor oxidoreductase; selenate reductase

Systematic name:

ferricytochrome c:selenite oxidoreductase

Comments:

The periplasmic enzyme from the bacterium Thauera selenatis is a complex comprising three heterologous subunits (α, β and γ) that contains a guanylyl molybdenum cofactor, as well as multiple iron-sulfur clusters and heme b. The enzyme reduces selenate to selenite using electrons provided by a diheme cytochrome c₄, which receives the electrons from the membrane quinone pool via EC 7.1.1.8, quinol—cytochrome-c reductase. Nitrate, nitrite, chlorate and sulfate are not substrates.

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, CAS registry number: 146359-71-9

References:

1.	Macy, J.M., Rech, S., Auling, G., Dorsch, M., Stackebrandt, E. and Sly, L.I. Thauera selenatis gen. nov., sp. nov., a member of the β subclass of Proteobacteria with a novel type of anaerobic respiration. Int. J. Syst. Bacteriol. 43 (1993) 135–142. [DOI] [PMID: 8427805]
2.	Schröder, I., Rech, S., Krafft, T. and Macy, J.M. Purification and characterization of the selenate reductase from Thauera selenatis. J. Biol. Chem. 272 (1997) 23765–23768. [DOI] [PMID: 9295321]
3.	Stolz, J.F. and Oremland, R.S. Bacterial respiration of arsenic and selenium. FEMS Microbiol. Rev. 23 (1999) 615–627. [DOI] [PMID: 10525169]
4.	Krafft, T., Bowen, A., Theis, F. and Macy, J.M. Cloning and sequencing of the genes encoding the periplasmic-cytochrome B-containing selenate reductase of Thauera selenatis. DNA 10 (2000) 365–377. [PMID: 10826693]
5.	Maher, M.J. and Macy, J.M. Crystallization and preliminary X-ray analysis of the selenate reductase from Thauera selenatis. Acta Crystallogr. D Biol. Crystallogr. 58 (2002) 706–708. [DOI] [PMID: 11914503]
6.	Maher, M.J., Santini, J., Pickering, I.J., Prince, R.C., Macy, J.M. and George, G.N. X-ray absorption spectroscopy of selenate reductase. Inorg. Chem. 43 (2004) 402–404. [DOI] [PMID: 14730999]
7.	Lowe, E.C., Bydder, S., Hartshorne, R.S., Tape, H.L., Dridge, E.J., Debieux, C.M., Paszkiewicz, K., Singleton, I., Lewis, R.J., Santini, J.M., Richardson, D.J. and Butler, C.S. Quinol-cytochrome c oxidoreductase and cytochrome c₄ mediate electron transfer during selenate respiration in Thauera selenatis. J. Biol. Chem. 285 (2010) 18433–18442. [DOI] [PMID: 20388716]

[EC 1.97.1.9 created 2003, modified 2024]