ExplorEnz: EC 2.1.1.179

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2.1.1.179

Accepted name:

16S rRNA (guanine¹⁴⁰⁵-N⁷)-methyltransferase

Reaction:

S-adenosyl-L-methionine + guanine¹⁴⁰⁵ in 16S rRNA = S-adenosyl-L-homocysteine + N⁷-methylguanine¹⁴⁰⁵ in 16S rRNA

Other name(s):

methyltransferase Sgm; m⁷G¹⁴⁰⁵ Mtase; Sgm Mtase; Sgm; sisomicin-gentamicin methyltransferase; sisomicin-gentamicin methylase; GrmA; RmtB; RmtC; ArmA

Systematic name:

S-adenosyl-L-methionine:16S rRNA (guanine¹⁴⁰⁵-N⁷)-methyltransferase

Comments:

The enzyme from the antibiotic-producing bacterium Micromonospora zionensis specifically methylates guanine¹⁴⁰⁵ at N⁷ in 16S rRNA, thereby rendering the ribosome resistant to 4,6-disubstituted deoxystreptamine aminoglycosides, which include gentamicins and kanamycins [2].

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB

References:

1.	Husain, N., Tkaczuk, K.L., Tulsidas, S.R., Kaminska, K.H., Cubrilo, S., Maravic-Vlahovicek, G., Bujnicki, J.M. and Sivaraman, J. Structural basis for the methylation of G¹⁴⁰⁵ in 16S rRNA by aminoglycoside resistance methyltransferase Sgm from an antibiotic producer: a diversity of active sites in m⁷G methyltransferases. Nucleic Acids Res. 38 (2010) 4120–4132. [DOI] [PMID: 20194115]
2.	Savic, M., Lovric, J., Tomic, T.I., Vasiljevic, B. and Conn, G.L. Determination of the target nucleosides for members of two families of 16S rRNA methyltransferases that confer resistance to partially overlapping groups of aminoglycoside antibiotics. Nucleic Acids Res. 37 (2009) 5420–5431. [DOI] [PMID: 19589804]
3.	Tomic, T.I., Moric, I., Conn, G.L. and Vasiljevic, B. Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site. Res. Microbiol. 159 (2008) 658–662. [DOI] [PMID: 18930134]
4.	Savic, M., Ilic-Tomic, T., Macmaster, R., Vasiljevic, B. and Conn, G.L. Critical residues for cofactor binding and catalytic activity in the aminoglycoside resistance methyltransferase Sgm. J. Bacteriol. 190 (2008) 5855–5861. [DOI] [PMID: 18586937]
5.	Maravic Vlahovicek, G., Cubrilo, S., Tkaczuk, K.L. and Bujnicki, J.M. Modeling and experimental analyses reveal a two-domain structure and amino acids important for the activity of aminoglycoside resistance methyltransferase Sgm. Biochim. Biophys. Acta 1784 (2008) 582–590. [DOI] [PMID: 18343347]
6.	Kojic, M., Topisirovic, L. and Vasiljevic, B. Cloning and characterization of an aminoglycoside resistance determinant from Micromonospora zionensis. J. Bacteriol. 174 (1992) 7868–7872. [DOI] [PMID: 1447159]
7.	Schmitt, E., Galimand, M., Panvert, M., Courvalin, P. and Mechulam, Y. Structural bases for 16 S rRNA methylation catalyzed by ArmA and RmtB methyltransferases. J. Mol. Biol. 388 (2009) 570–582. [DOI] [PMID: 19303884]
8.	Wachino, J., Shibayama, K., Kimura, K., Yamane, K., Suzuki, S. and Arakawa, Y. RmtC introduces G¹⁴⁰⁵ methylation in 16S rRNA and confers high-level aminoglycoside resistance on Gram-positive microorganisms. FEMS Microbiol. Lett. 311 (2010) 56–60. [DOI] [PMID: 20722735]
9.	Liou, G.F., Yoshizawa, S., Courvalin, P. and Galimand, M. Aminoglycoside resistance by ArmA-mediated ribosomal 16S methylation in human bacterial pathogens. J. Mol. Biol. 359 (2006) 358–364. [DOI] [PMID: 16626740]

[EC 2.1.1.179 created 2010]