ExplorEnz: EC 2.1.1.188

Your query returned 1 entry. Printable version

2.1.1.188

Accepted name:

23S rRNA (guanine⁷⁴⁸-N¹)-methyltransferase

Reaction:

S-adenosyl-L-methionine + guanine⁷⁴⁸ in 23S rRNA = S-adenosyl-L-homocysteine + N¹-methylguanine⁷⁴⁸ in 23S rRNA

Other name(s):

Rlma(II); Rlma2; 23S rRNA m¹G⁷⁴⁸ methyltransferase; RlmaII; Rlma II; tylosin-resistance methyltransferase RlmA(II); TlrB; rRNA large subunit methyltransferase II

Systematic name:

S-adenosyl-L-methionine:23S rRNA (guanine⁷⁴⁸-N¹)-methyltransferase

Comments:

The enzyme specifically methylates guanine⁷⁴⁸ at N¹ in 23S rRNA. The methyltransferase RlmA^II confers resistance to the macrolide antibiotic tylosin in the drug-producing strain Streptomyces fradiae [1].

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc

References:

1.	Douthwaite, S., Crain, P.F., Liu, M. and Poehlsgaard, J. The tylosin-resistance methyltransferase RlmA^II (TlrB) modifies the N-1 position of 23S rRNA nucleotide G⁷⁴⁸. J. Mol. Biol. 337 (2004) 1073–1077. [DOI] [PMID: 15046978]
2.	Liu, M., Kirpekar, F., Van Wezel, G.P. and Douthwaite, S. The tylosin resistance gene tlrB of Streptomyces fradiae encodes a methyltransferase that targets G⁷⁴⁸ in 23S rRNA. Mol. Microbiol. 37 (2000) 811–820. [DOI] [PMID: 10972803]
3.	Lebars, I., Yoshizawa, S., Stenholm, A.R., Guittet, E., Douthwaite, S. and Fourmy, D. Structure of 23S rRNA hairpin 35 and its interaction with the tylosin-resistance methyltransferase RlmA^II. EMBO J. 22 (2003) 183–192. [DOI] [PMID: 12514124]
4.	Lebars, I., Husson, C., Yoshizawa, S., Douthwaite, S. and Fourmy, D. Recognition elements in rRNA for the tylosin resistance methyltransferase RlmA^II. J. Mol. Biol. 372 (2007) 525–534. [DOI] [PMID: 17673230]
5.	Douthwaite, S., Jakobsen, L., Yoshizawa, S. and Fourmy, D. Interaction of the tylosin-resistance methyltransferase RlmA^II at its rRNA target differs from the orthologue RlmA^I. J. Mol. Biol. 378 (2008) 969–975. [DOI] [PMID: 18406425]
6.	Liu, M. and Douthwaite, S. Methylation at nucleotide G⁷⁴⁵ or G⁷⁴⁸ in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria. Mol. Microbiol. 44 (2002) 195–204. [DOI] [PMID: 11967079]

[EC 2.1.1.188 created 1976 as EC 2.1.1.51, part transferred 2010 to EC 2.1.1.188]