ExplorEnz: EC 2.1.1.220

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2.1.1.220

Accepted name:

tRNA (adenine⁵⁸-N¹)-methyltransferase

Reaction:

S-adenosyl-L-methionine + adenine⁵⁸ in tRNA = S-adenosyl-L-homocysteine + N¹-methyladenine⁵⁸ in tRNA

Other name(s):

tRNA m¹A⁵⁸ methyltransferase; tRNA (m¹A⁵⁸) methyltransferase; TrmI; tRNA (m¹A⁵⁸) Mtase; Rv2118cp; Gcd10p-Gcd14p; Trm61p-Trm6p

Systematic name:

S-adenosyl-L-methionine:tRNA (adenine⁵⁸-N¹)-methyltransferase

Comments:

The enzyme specifically methylates adenine⁵⁸ in tRNA. The methylation of A58 is critical for maintaining the stability of initiator tRNA^Met in yeast [3].

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB

References:

1.	Droogmans, L., Roovers, M., Bujnicki, J.M., Tricot, C., Hartsch, T., Stalon, V. and Grosjean, H. Cloning and characterization of tRNA (m¹A⁵⁸) methyltransferase (TrmI) from Thermus thermophilus HB27, a protein required for cell growth at extreme temperatures. Nucleic Acids Res. 31 (2003) 2148–2156. [PMID: 12682365]
2.	Varshney, U., Ramesh, V., Madabushi, A., Gaur, R., Subramanya, H.S. and RajBhandary, U.L. Mycobacterium tuberculosis Rv2118c codes for a single-component homotetrameric m¹A⁵⁸ tRNA methyltransferase. Nucleic Acids Res. 32 (2004) 1018–1027. [DOI] [PMID: 14960715]
3.	Anderson, J., Phan, L. and Hinnebusch, A.G. The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 97 (2000) 5173–5178. [DOI] [PMID: 10779558]

[EC 2.1.1.220 created 2011 (EC 2.1.1.36 created 1972, part transferred 2011 to EC 2.1.1.220)]