ExplorEnz: EC 2.1.1.260

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2.1.1.260

Accepted name:

rRNA small subunit pseudouridine methyltransferase Nep1

Reaction:

S-adenosyl-L-methionine + pseudouridine¹¹⁹¹ in yeast 18S rRNA = S-adenosyl-L-homocysteine + N¹-methylpseudouridine¹¹⁹¹ in yeast 18S rRNA

Other name(s):

Nep1; nucleolar essential protein 1

Systematic name:

S-adenosyl-L-methionine:18S rRNA (pseudouridine¹¹⁹¹-N¹)-methyltransferase

Comments:

This enzyme, which occurs in both prokaryotes and eukaryotes, recognizes specific pseudouridine residues (Ψ) in small subunits of ribosomal RNA based on the local RNA structure. It recognizes Ψ⁹¹⁴ in 16S rRNA from the archaeon Methanocaldococcus jannaschii, Ψ¹¹⁹¹ in yeast 18S rRNA, and Ψ¹²⁴⁸ in human 18S rRNA.

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB

References:

1.	Taylor, A.B., Meyer, B., Leal, B.Z., Kötter, P., Schirf, V., Demeler, B., Hart, P.J., Entian, K.-D. and Wöhnert, J. The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site. Nucleic Acids Res. 36 (2008) 1542–1554. [DOI] [PMID: 18208838]
2.	Wurm, J.P., Meyer, B., Bahr, U., Held, M., Frolow, O., Kötter, P., Engels, J.W., Heckel, A., Karas, M., Entian, K.-D. and Wöhnert, J. The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N¹-specific methyltransferase. Nucleic Acids Res. 38 (2010) 2387–2398. [DOI] [PMID: 20047967]
3.	Meyer, B., Wurm, J.P., Kötter, P., Leisegang, M.S., Schilling, V., Buchhaupt, M., Held, M., Bahr, U., Karas, M., Heckel, A., Bohnsack, M.T., Wöhnert, J. and Entian, K.-D. The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ¹¹⁹¹ in yeast 18S rRNA. Nucleic Acids Res. 39 (2011) 1526–1537. [DOI] [PMID: 20972225]

[EC 2.1.1.260 created 2012]