ExplorEnz: EC 2.1.1.35

Your query returned 1 entry. Printable version

2.1.1.35

Accepted name:

tRNA (uracil⁵⁴-C⁵)-methyltransferase

Reaction:

S-adenosyl-L-methionine + uracil⁵⁴ in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil⁵⁴ in tRNA

Other name(s):

transfer RNA uracil⁵⁴ 5-methyltransferase; transfer RNA uracil⁵⁴ methylase; tRNA uracil⁵⁴ 5-methyltransferase; m⁵U⁵⁴-methyltransferase; tRNA:m⁵U⁵⁴-methyltransferase; RUMT; TrmA; 5-methyluridine⁵⁴ tRNA methyltransferase; tRNA(uracil-54,C⁵)-methyltransferase; Trm2; tRNA(m⁵U⁵⁴)methyltransferase

Systematic name:

S-adenosyl-L-methionine:tRNA (uracil⁵⁴-C⁵)-methyltransferase

Comments:

Unlike this enzyme, EC 2.1.1.74 (methylenetetrahydrofolate—tRNA-(uracil⁵⁴-C⁵)-methyltransferase (FADH₂-oxidizing)), uses 5,10-methylenetetrahydrofolate and FADH₂ to supply the atoms for methylation of U⁵⁴ [4].

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 37257-02-6

References:

1.	Björk, G.R. and Svensson, I. Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae. Eur. J. Biochem. 9 (1969) 207–215. [DOI] [PMID: 4896260]
2.	Greenberg, R. and Dudock, B. Isolation and characterization of m5U-methyltransferase from Escherichia coli. J. Biol. Chem. 255 (1980) 8296–8302. [PMID: 6997293]
3.	Hurwitz, J., Gold, M. and Anders, M. The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. 3. Purification of soluble ribonucleic acid-methylating enzymes. J. Biol. Chem. 239 (1964) 3462–3473. [PMID: 14245404]
4.	Delk, A.S., Nagle, D.P., Jr. and Rabinowitz, J.C. Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH₂. J. Biol. Chem. 255 (1980) 4387–4390. [PMID: 6768721]
5.	Kealey, J.T., Gu, X. and Santi, D.V. Enzymatic mechanism of tRNA (m⁵U⁵⁴)methyltransferase. Biochimie 76 (1994) 1133–1142. [DOI] [PMID: 7748948]
6.	Gu, X., Ivanetich, K.M. and Santi, D.V. Recognition of the T-arm of tRNA by tRNA (m⁵U54)-methyltransferase is not sequence specific. Biochemistry 35 (1996) 11652–11659. [DOI] [PMID: 8794745]
7.	Becker, H.F., Motorin, Y., Sissler, M., Florentz, C. and Grosjean, H. Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the TΨ-loop of yeast tRNAs. J. Mol. Biol. 274 (1997) 505–518. [DOI] [PMID: 9417931]
8.	Walbott, H., Leulliot, N., Grosjean, H. and Golinelli-Pimpaneau, B. The crystal structure of Pyrococcus abyssi tRNA (uracil-54, C⁵)-methyltransferase provides insights into its tRNA specificity. Nucleic Acids Res. 36 (2008) 4929–4940. [DOI] [PMID: 18653523]

[EC 2.1.1.35 created 1972, modified 2011]