ExplorEnz: EC 2.1.1.405

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2.1.1.405

Accepted name:

5-hydroxybenzimidazole ribotide O-methyltransferase

Reaction:

S-adenosyl-L-methionine + 5-hydroxybenzimidazole ribotide = S-adenosyl-L-homocysteine + 5-methoxybenzimidazole ribotide

Glossary:

5-hydroxybenzimidazole ribotide = N¹-(α-D-ribosyl)-5-hydroxybenzimidazole
5-methoxybenzimidazole ribotide = N¹-(α-D-ribosyl)-5-methoxybenzimidazole

Other name(s):

bzaC (gene name)

Systematic name:

5-hydroxybenzimidazole ribotide O-methyltransferase

Comments:

The enzyme, characterized from the bacteria Eubacterium limosum and Moorella thermoacetica, participates in an anaerobic pathway for the biosynthesis the lower ligand used in cobalamin. Different species produce different lower ligands (i.e., 5-hydroxybenzimidazole, 5-methoxybenzimidazole, 5-methoxy-6-methylbenzimidazole, or 5,6-dimethylbenzimidazole).

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Hazra, A.B., Han, A.W., Mehta, A.P., Mok, K.C., Osadchiy, V., Begley, T.P. and Taga, M.E. Anaerobic biosynthesis of the lower ligand of vitamin B₁₂. Proc. Natl. Acad. Sci. USA 112 (2015) 10792–10797. [DOI] [PMID: 26246619]
2.	Mathur, Y., Sreyas, S., Datar, P.M., Sathian, M.B. and Hazra, A.B. CobT and BzaC catalyze the regiospecific activation and methylation of the 5-hydroxybenzimidazole lower ligand in anaerobic cobamide biosynthesis. J. Biol. Chem. 295 (2020) 10522–10534. [DOI] [PMID: 32503839]

[EC 2.1.1.405 created 2025]