ExplorEnz: EC 2.3.1.204

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2.3.1.204

Accepted name:

lipoyl-[GcvH]:protein N-lipoyltransferase

Reaction:

[glycine cleavage system H protein]-N⁶-dihydrolipoyl-L-lysine + a [lipoyl-carrier protein] = [glycine cleavage system H protein] + a [lipoyl-carrier protein]-N⁶-dihydrolipoyl-L-lysine

Glossary:

glycine cleavage system H protein = GcvH
lipoic acid = 5-[(3R)-1,2-dithiolan-3-yl]pentanoic acid

Other name(s):

LipL; octanoyl-[GcvH]:E2 amidotransferase; ywfL (gene name); octanoyl-[GcvH]:protein N-octanoyltransferase; [glycine cleavage system H]-N⁶-octanoyl-L-lysine:[lipoyl-carrier protein]-N⁶-L-lysine octanoyltransferase

Systematic name:

[glycine cleavage system H protein]-N⁶-dihydrolipoyl-L-lysine:[lipoyl-carrier protein]-N⁶-L-lysine dihydrolipoyltransferase

Comments:

The enzyme has been shown in multiple bacteria to catalyse the amidotransfer of the dihydrolipoyl moiety from either the H protein of the glycine cleavage system (EC 1.4.1.27) or the E2 component (dihydrolipoamide acetyltransferase) of the 2-oxoglutarate dehydrogenase system (EC 1.2.1.105) to the E2 component of other 2-oxoacid dehydrogenase systems: the pyruvate dehydrogenase system (EC 1.2.1.104), the branched-chain α-keto acid dehydrogenase system (EC 1.2.1.25), and the acetoin dehydrogenase system (EC 2.3.1.190).

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB

References:

1.	Christensen, Q.H., Martin, N., Mansilla, M.C., de Mendoza, D. and Cronan, J.E. A novel amidotransferase required for lipoic acid cofactor assembly in Bacillus subtilis. Mol. Microbiol. 80 (2011) 350–363. [DOI] [PMID: 21338421]
2.	Christensen, Q.H., Hagar, J.A., O'Riordan, M.X. and Cronan, J.E. A complex lipoate utilization pathway in Listeria monocytogenes. J. Biol. Chem. 286 (2011) 31447–31456. [DOI] [PMID: 21768091]
3.	Martin, N., Christensen, Q.H., Mansilla, M.C., Cronan, J.E. and de Mendoza, D. A novel two-gene requirement for the octanoyltransfer reaction of Bacillus subtilis lipoic acid biosynthesis. Mol. Microbiol. 80 (2011) 335–349. [DOI] [PMID: 21338420]
4.	Rasetto, N.B., Lavatelli, A., Martin, N. and Mansilla, M.C. Unravelling the lipoyl-relay of exogenous lipoate utilization in Bacillus subtilis. Mol. Microbiol. 112 (2019) 302–316. [DOI] [PMID: 31066113]
5.	Cronan, J.E. Lipoic acid attachment to proteins: stimulating new developments. Microbiol. Mol. Biol. Rev. 88:e0000524 (2024). [DOI] [PMID: 38624243]

[EC 2.3.1.204 created 2012, modified 2025 (EC 2.3.1.200 created 2012, incorporated 2026), modified 2026]