ExplorEnz: EC 2.3.1.234

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2.3.1.234

Accepted name:

N⁶-L-threonylcarbamoyladenine synthase

Reaction:

L-threonylcarbamoyladenylate + adenine³⁷ in tRNA = AMP + N⁶-L-threonylcarbamoyladenine³⁷ in tRNA

For diagram of N⁶-L-threonylcarbamoyladenosine³⁷ modified tRNA biosynthesis, click here

Glossary:

N⁶-L-threonylcarbamoyladenine³⁷ = t6A37

Other name(s):

t6A synthase; Kae1; ygjD (gene name); Qri7

Systematic name:

L-threonylcarbamoyladenylate:adenine³⁷ in tRNA N⁶-L-threonylcarbamoyltransferase

Comments:

The enzyme is involved in the synthesis of N⁶-threonylcarbamoyladenosine³⁷ in tRNAs, which is found in tRNAs with the anticodon NNU, i.e. tRNA^Ile, tRNA^Thr, tRNA^Asn, tRNA^Lys, tRNA^Ser and tRNA^Arg [3].

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB

References:

1.	Lauhon, C.T. Mechanism of N⁶-threonylcarbamoyladenonsine (t⁶A) biosynthesis: isolation and characterization of the intermediate threonylcarbamoyl-AMP. Biochemistry 51 (2012) 8950–8963. [DOI] [PMID: 23072323]
2.	Deutsch, C., El Yacoubi, B., de Crecy-Lagard, V. and Iwata-Reuyl, D. Biosynthesis of threonylcarbamoyl adenosine (t⁶A), a universal tRNA nucleoside. J. Biol. Chem. 287 (2012) 13666–13673. [DOI] [PMID: 22378793]
3.	Perrochia, L., Crozat, E., Hecker, A., Zhang, W., Bareille, J., Collinet, B., van Tilbeurgh, H., Forterre, P. and Basta, T. In vitro biosynthesis of a universal t⁶A tRNA modification in Archaea and Eukarya. Nucleic Acids Res. 41 (2013) 1953–1964. [DOI] [PMID: 23258706]
4.	Wan, L.C.K., Mao, D.Y.L., Neculai, D., Strecker, J., Chiovitti, D., Kurinov, I., Poda, G., Thevakumaran, N., Yuan, F., Szilard, R.K., Lissina, E., Nislow, C., Caudy, A.A., Durocher, D. and Sicheri, F. Reconstitution and characterization of eukaryotic N⁶-threonylcarbamoylation of tRNA using a minimal enzyme system. Nucleic Acids Res. 41 (2013) 6332–6346. [DOI] [PMID: 23620299]

[EC 2.3.1.234 created 2014 as EC 2.6.99.4, transferred 2014 to EC 2.3.1.234]