EC |
2.4.1.230 |
Accepted name: |
kojibiose phosphorylase |
Reaction: |
2-α-D-glucosyl-D-glucose + phosphate = D-glucose + β-D-glucose 1-phosphate |
Systematic name: |
2-α-D-glucosyl-D-glucose:phosphate β-D-glucosyltransferase |
Comments: |
The enzyme from Thermoanaerobacter brockii can act with α-1,2-oligoglucans, such as selaginose, as substrate, but more slowly. The enzyme is inactive when dissaccharides with linkages other than α-1,2 linkages, such as sophorose, trehalose, neotrehalose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, gentiobiose, sucrose and lactose, are used as substrates. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 206566-36-1 |
References: |
1. |
Chaen, H., Yamamoto, T., Nishimoto, T., Nakada, T., Fukuda, S., Sugimoto, T., Kurimoto, M. and Tsujisaka, Y. Purification and characterization of a novel phosphorylase, kojibiose phosphorylase, from Thermoanaerobium brockii. J. Appl. Glycosci. 46 (1999) 423–429. |
2. |
Chaen, H., Nishimoto, T., Nakada, T., Fukuda, S., Kurimoto, M. and Tsujisaka, Y. Enzymatic synthesis of kojioligosaccharides using kojibiose phosphorylase. J. Biosci. Bioeng. 92 (2001) 177–182. [DOI] [PMID: 16233080] |
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[EC 2.4.1.230 created 2003] |
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