ExplorEnz: EC 2.4.2.2

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2.4.2.2

Accepted name:

pyrimidine-nucleoside phosphorylase

Reaction:

(1) uridine + phosphate = uracil + α-D-ribose 1-phosphate
(2) cytidine + phosphate = cytosine + α-D-ribose 1-phosphate
(3) 2′-deoxyuridine + phosphate = uracil + 2-deoxy-α-D-ribose 1-phosphate
(4) thymidine + phosphate = thymine + 2-deoxy-α-D-ribose 1-phosphate

Other name(s):

Py-NPase; pdp (gene name)

Systematic name:

pyrimidine-nucleoside:phosphate (2′-deoxy)-α-D-ribosyltransferase

Comments:

Unlike EC 2.4.2.3, uridine phosphorylase, and EC 2.4.2.4, thymidine phosphorylase, this enzyme can accept both the ribonucleosides uridine and cytidine and the 2′-deoxyribonucleosides 2′-deoxyuridine and thymidine [3,6]. The reaction is reversible, and the enzyme does not distinguish between α-D-ribose 1-phosphate and 2-deoxy-α-D-ribose 1-phosphate in the synthetic direction.

Links to other databases:

BRENDA, EXPASY, Gene, GTD, KEGG, MetaCyc, PDB, CAS registry number: 9055-35-0

References:

1.	Friedkin, M. and Kalckar, H. Nucleoside phosphorylases. In: Boyer, P.D., Lardy, H. and Myrbäck, K. (Ed.), The Enzymes, 2nd edn, vol. 5, Academic Press, New York, 1961, pp. 237–255.
2.	Saunders, P.P., Wilson, B.A. and Saunders, G.F. Purification and comparative properties of a pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus. J. Biol. Chem. 244 (1969) 3691–3697. [PMID: 4978445]
3.	Hamamoto, T., Noguchi, T. and Midorikawa, Y. Purification and characterization of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus TH 6-2. Biosci. Biotechnol. Biochem. 60 (1996) 1179–1180. [DOI] [PMID: 8782414]
4.	Okuyama, K., Hamamoto, T., Noguchi, T. and Midorikawa, Y. Molecular cloning and expression of the pyrimidine nucleoside phosphorylase gene from Bacillus stearothermophilus TH 6-2. Biosci. Biotechnol. Biochem. 60 (1996) 1655–1659. [DOI] [PMID: 8987664]
5.	Pugmire, M.J. and Ealick, S.E. The crystal structure of pyrimidine nucleoside phosphorylase in a closed conformation. Structure 6 (1998) 1467–1479. [DOI] [PMID: 9817849]
6.	Wei, X.K., Ding, Q.B., Zhang, L., Guo, Y.L., Ou, L. and Wang, C.L. Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside. J Zhejiang Univ Sci B 9 (2008) 520–526. [DOI] [PMID: 18600781]

[EC 2.4.2.2 created 1961, modified 2021]